Identification of reference genes suitable for qRT-PCR in grapevine and application for the study of the expression of genes involved in pterostilbene synthesis
| العنوان: | Identification of reference genes suitable for qRT-PCR in grapevine and application for the study of the expression of genes involved in pterostilbene synthesis |
|---|---|
| المؤلفون: | Jani Kelloniemi, David Wendehenne, Benoît Poinssot, Marie-Claire Héloir, Marielle Adrian, Magdalena Gamm |
| المساهمون: | Plante - microbe - environnement : biochimie, biologie cellulaire et écologie (PMEBBCE), Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD) |
| المصدر: | Molecular Genetics and Genomics Molecular Genetics and Genomics, Springer Verlag, 2011, 285 (4), pp.273-285. ⟨10.1007/s00438-011-0607-2⟩ |
| بيانات النشر: | Springer Science and Business Media LLC, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | 0106 biological sciences, Pterostilbene, [SDV]Life Sciences [q-bio], Statistics as Topic, reference gene, Phenylalanine ammonia-lyase, resveratrol, Genes, Plant, o-methyltransferase, 01 natural sciences, 03 medical and health sciences, chemistry.chemical_compound, Gene Expression Regulation, Plant, Reference genes, Stilbenes, Gene expression, Genetics, Vitis, Molecular Biology, Gene, DNA Primers, 030304 developmental biology, Botrytis cinerea, 2. Zero hunger, Regulation of gene expression, 0303 health sciences, biology, Reverse Transcriptase Polymerase Chain Reaction, Reproducibility of Results, General Medicine, Reference Standards, biology.organism_classification, grapevine, 3. Good health, Biochemistry, chemistry, Plasmopara viticola, plasmopara viticola, Software, 010606 plant biology & botany |
| الوصف: | International audience; The recent publication of the grapevine genome sequence facilitates the use of qRT-PCR to study gene expression changes. For this approach, reference genes are commonly used to normalize data and their stability of expression should be systematically validated. Among grapevine defenses is the production of the antimicrobial stilbenic phytoalexins, notably the highly fungitoxic pterostilbene, which plays a crucial role in grapevine interaction with Plasmopara viticola and Botrytis cinerea. As a resveratrol O-methyltransferase (ROMT) gene involved in pterostilbene synthesis was recently identified, we investigated the accumulation of the corresponding transcripts to those of two other stilbene biosynthesis related genes phenylalanine ammonia lyase (PAL) and stilbene synthase (STS) in response to pathogen infection. Using three computer-based statistical methods and C-t values or LRE method generated values as input data, we have first identified two reference genes (VATP16 and 60SRP) suitable for normalization of qPCR expression data obtained in grapevine leaves and berries infected by P. viticola and B. cinerea, respectively. Next, we have highlighted that the expression of ROMT is induced in P. viticola-infected leaves and also in B. cinerea-infected berries, confirming the involvement of pterostilbene in grapevine defenses. |
| تدمد: | 1617-4623 1617-4615 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9af9846a3532bb8adb9d33596731a74fTest https://doi.org/10.1007/s00438-011-0607-2Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....9af9846a3532bb8adb9d33596731a74f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16174623 16174615 |
|---|