Antagonism of betulinic acid on LPS-mediated inhibition of ABCA1 and cholesterol efflux through inhibiting nuclear factor-kappaB signaling pathway and miR-33 expression
| العنوان: | Antagonism of betulinic acid on LPS-mediated inhibition of ABCA1 and cholesterol efflux through inhibiting nuclear factor-kappaB signaling pathway and miR-33 expression |
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| المؤلفون: | Shi-Lin Tang, Feng Yao, Ping-Ping He, Yun-Cheng Lv, Chao-Ke Tang, Xin-Ping Ouyang, Yan-Yan Tang, Wu-Jun Chen, Kai Yin, Yuchang Fu, Qian Lu, Min Zhang, Da-Wei Zhang, Guo-Jun Zhao |
| المصدر: | PLoS ONE, Vol 8, Iss 9, p e74782 (2013) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | miR-33, Lipopolysaccharides, Male, lcsh:Medicine, Biology, Models, Biological, Cell Line, chemistry.chemical_compound, Mice, Apolipoproteins E, Betulinic acid, Animals, Humans, Betulinic Acid, lcsh:Science, Regulation of gene expression, Cell Nucleus, Multidisciplinary, Macrophages, Body Weight, lcsh:R, NF-kappa B, Biological Transport, Atherosclerosis, Molecular biology, Lipids, Triterpenes, Cell biology, MicroRNAs, Protein Transport, ATP Binding Cassette Transporter 1, Cholesterol, chemistry, Gene Expression Regulation, ABCA1, biology.protein, Phosphorylation, lipids (amino acids, peptides, and proteins), lcsh:Q, Efflux, Signal transduction, Pentacyclic Triterpenes, Research Article, Signal Transduction |
| الوصف: | ATP-binding cassette transporter A1 (ABCA1) is critical in exporting cholesterol from macrophages and plays a protective role in the development of atherosclerosis. The purpose of this study was to investigate the effects of betulinic acid (BA), a pentacyclic triterpenoid, on ABCA1 expression and cholesterol efflux, and to further determine the underlying mechanism. BA promoted ABCA1 expression and cholesterol efflux, decreased cellular cholesterol and cholesterol ester content in LPS-treated macrophages. Furthermore, we found that BA promoted ABCA1 expression via down-regulation of miR-33s. The inhibition of LPS-induced NF-κB activation further decreased miR-33s expression and enhanced ABCA1 expression and cholesterol efflux when compared with BA only treatment. In addition, BA suppressed IκB phosphorylation, p65 phosphorylation and nuclear translocation, and the transcription of NF-κB-dependent related gene. Moreover, BA reduced atherosclerotic lesion size, miR-33s levels and NF-κB activation, and promoted ABCA1 expression in apoE(-/-) mice. Taken together, these results reveal a novel mechanism for the BA-mediated ABCA1 expression, which may provide new insights for developing strategies for modulating vascular inflammation and atherosclerosis. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9abd28344eee39e5c80e546bc88327bfTest http://europepmc.org/articles/PMC3783495?pdf=renderTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9abd28344eee39e5c80e546bc88327bf |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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