The differentially DNA-methylated region responsible for expression of runt-related transcription factor 2
| العنوان: | The differentially DNA-methylated region responsible for expression of runt-related transcription factor 2 |
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| المؤلفون: | Yuichi Hidaka, Daigo Yokoi, Shoichi Wakitani, Koichiro Nishino |
| المصدر: | The Journal of Veterinary Medical Science |
| بيانات النشر: | Japanese Society of Veterinary Science, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Male, musculoskeletal diseases, 0301 basic medicine, correlation analysis, Response element, Bone Marrow Cells, Core Binding Factor Alpha 1 Subunit, Biology, osteogenesis, 03 medical and health sciences, Dogs, Epigenetics of physical exercise, Species Specificity, stomatognathic system, Runx2, Transcription (biology), Animals, Promoter Regions, Genetic, Transcription factor, Cells, Cultured, DNA methylation, promoter, Osteoblasts, Full Paper, General Veterinary, musculoskeletal, neural, and ocular physiology, Cell Differentiation, Promoter, Methylation, musculoskeletal system, Molecular biology, Mice, Inbred C57BL, 030104 developmental biology, Differentially methylated regions, Gene Expression Regulation, embryonic structures, Anatomy |
| الوصف: | Runt-related transcription factor 2 (Runx2) is essential for osteogenesis. This study aimes at identification of the genomic region differentially methylated in DNA for regulation of Runx2 expression. In the proximal promoter of mouse Runx2, DNA methylation was frequent at the region further than 3 kb relative to the transcription start site, in contrast to lower methylation status of the closer locus within 2 kb from the transcription start site. At the intermediate part, we identified a novel differentially methylated region in the Runx2 promoter region (Runx2-DMR): from -2.7 to -2.2 kb relative to the start site of Runx2 transcription in mice. In this region, the DNA methylation rate correlated negatively with Runx2 expression among mouse organs as well as among primary cultures of bone marrow from different dogs. Induction of mouse and dog mesenchymal-like cells into osteoblastic differentiation decreased the methylation rate of Runx2-DMR. Thus, in this study, we identified a novel genomic region in which DNA methylation status is related to Runx2 expression and detected demethylation of Runx2-DMR during osteoblastic differentiation in mouse and dog. |
| تدمد: | 1347-7439 0916-7250 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9955e2b11d79ce6c69fb546f0fc1e48dTest https://doi.org/10.1292/jvms.16-0321Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9955e2b11d79ce6c69fb546f0fc1e48d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13477439 09167250 |
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