Genomic and proteomic analysis of transcription factor TFII-I reveals insight into the response to cellular stress
| العنوان: | Genomic and proteomic analysis of transcription factor TFII-I reveals insight into the response to cellular stress |
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| المؤلفون: | Giorgio L. Papadopoulos, Rolf Renne, Tommy Ming Tang, Mir A. Hossain, Jörg Bungert, Jianhong Hu, John Strouboulis, Alex Xiu-Cheng Fan, I.-Ju Lin, Michael S. Kilberg |
| المصدر: | Fan, A X, Papadopoulos, G L, Hossain, M A, Lin, I-J, Hu, J, Tang, T M, Kilberg, M S, Renne, R, Strouboulis, J & Bungert, J 2014, ' Genomic and proteomic analysis of transcription factor TFII-I reveals insight into the response to cellular stress ', Nucleic Acids Research, vol. 42, no. 12, pp. 7625-7641 . https://doi.org/10.1093/nar/gku467Test Nucleic Acids Research |
| بيانات النشر: | Oxford University Press (OUP), 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Proteomics, Elongin, Response element, Carbon-Nitrogen Ligases/metabolism, RNA polymerase II, Transcription Factors, TFII, 03 medical and health sciences, 0302 clinical medicine, Stress, Physiological, Genetics, Humans, Carbon-Nitrogen Ligases, Biotinylation, Transcription Factors/metabolism, Enhancer, Nuclear Proteins/metabolism, 030304 developmental biology, TATA-Binding Protein Associated Factors, 0303 health sciences, Activating Transcription Factor 3, Binding Sites, biology, General transcription factor, Escherichia coli Proteins, Repressor Proteins/metabolism, Gene regulation, Chromatin and Epigenetics, Nuclear Proteins, Escherichia coli Proteins/metabolism, Promoter, Genomics, TATA-Binding Protein Associated Factors/metabolism, Molecular biology, Repressor Proteins, DNA Topoisomerases, Type II, Transcription Factors, TFII/metabolism, Activating Transcription Factor 3/genetics, 030220 oncology & carcinogenesis, Transcription preinitiation complex, biology.protein, RNA Polymerase II, DNA Topoisomerases, Type II/metabolism, Transcription Initiation Site, Transcription factor II D, K562 Cells, RNA Polymerase II/metabolism, Transcription factor II B, Transcription Factors, Stress, Physiological/genetics |
| الوصف: | The ubiquitously expressed transcription factor TFII-I exerts both positive and negative effects on transcription. Using biotinylation tagging technology and high-throughput sequencing, we determined sites of chromatin interactions for TFII-I in the human erythroleukemia cell line K562. This analysis revealed that TFII-I binds upstream of the transcription start site of expressed genes, both upstream and downstream of the transcription start site of repressed genes, and downstream of RNA polymerase II peaks at the ATF3 and other stress responsive genes. At the ATF3 gene, TFII-I binds immediately downstream of a Pol II peak located 5 kb upstream of exon 1. Induction of ATF3 expression increases transcription throughout the ATF3 gene locus which requires TFII-I and correlates with increased association of Pol II and Elongin A. Pull-down assays demonstrated that TFII-I interacts with Elongin A. Partial depletion of TFII-I expression caused a reduction in the association of Elongin A with and transcription of the DNMT1 and EFR3A genes without a decrease in Pol II recruitment. The data reveal different interaction patterns of TFII-I at active, repressed, or inducible genes, identify novel TFII-I interacting proteins, implicate TFII-I in the regulation of transcription elongation and provide insight into the role of TFII-I during the response to cellular stress. |
| وصف الملف: | application/pdf |
| تدمد: | 1362-4962 0305-1048 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9873cb07d05a57bf6887f18b966e17c4Test https://doi.org/10.1093/nar/gku467Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9873cb07d05a57bf6887f18b966e17c4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13624962 03051048 |
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