Different types of dietary fibers trigger specific alterations in composition and predicted functions of colonic bacterial communities in BALB/c mice
| العنوان: | Different types of dietary fibers trigger specific alterations in composition and predicted functions of colonic bacterial communities in BALB/c mice |
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| المؤلفون: | Cai Jingyi, Hauke Smidt, Gang Tian, Qiufeng Zeng, Hua Li, Jian Li, Jianping Wang, Ling Li Zhang, Shiping Bai, André-Denis G. Wright, Daiwen Chen, Yuheng Luo, Xuemei Ding, Keying Zhang, Ping Zheng |
| المصدر: | Frontiers in Microbiology Frontiers in Microbiology, 8 Frontiers in Microbiology, Vol 8 (2017) Frontiers in Microbiology 8 (2017) |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Microbiology (medical), 030106 microbiology, lcsh:QR1-502, Carbohydrate metabolism, Polysaccharide, Microbiology, lcsh:Microbiology, 16S high throughput sequencing, 03 medical and health sciences, PICRUSt predicted functions, Microbiologie, Prevotella, BALB/c mice, Original Research, VLAG, chemistry.chemical_classification, biology, Carbohydrate, Dietary fibers, biology.organism_classification, Enterobacteriaceae, 030104 developmental biology, chemistry, Colonic bacterial community, Bacteroides, Proteobacteria, Bacteria |
| الوصف: | Soluble dietary fibers (SDF) are fermented more than insoluble dietary fibers (IDF), but their effect on colonic bacterial community structure and function remains unclear. Thus, bacterial community composition and function in the colon of BALB/c mice (n = 7) fed with a high level (approximately 20%) of typical SDF, oat-derived β-glucan (G), microcrystalline cellulose (M) as IDF, or their mixture (GM), were compared. Mice in group G showed a lowest average feed intake (p < 0.05) but no change on the average body weight gain (p > 0.05) compared to other groups, which may be associated with the highest concentration of colonic propionate (p < 0.05) in these mice. The bacterial α-diversity of group G was significantly lower than other groups (p < 0.01). In group G, the relative abundance of bacteria belonging to the phylum Bacteroidetes was significantly increased, whereas bacteria from the phylum Firmicutes were significantly decreased (p < 0.01). The core bacteria for different treatments showed distinct differences. Bacteroides, Dehalobacterium, and Prevotella, including known acetogens and carbohydrate fermenting organisms, were significantly increased in relative abundance in group G. In contrast, Adlercreutzia, Odoribacter, and Coprococcus were significantly more abundant in group M, whereas Oscillospira, Desulfovibrio, and Ruminoccaceae, typical hydrogenotrophs equipped with multiple carbohydrate active enzymes, were remarkably enriched in group GM (p < 0.05). The relative abundance of bacteria from the three classes of Proteobacteria, Betaproteobacteria, Gammaproteobacteria (including Enterobacteriaceae) and Deltaproteobacteria, were significantly more abundant in group G, indicating a higher ratio of conditional pathogenic bacteria in mice fed dietary β-glucan in current study. The predicted colonic microbial function showed an enrichment of “Energy metabolism” and “Carbohydrate metabolism” pathways in mice from group G and M, suggesting that the altered bacterial community in the colon of mice with the two dietary fibers probably resulted in a more efficient degradation of dietary polysaccharides. Our result suggests that the influence of dietary β-glucan (SDF) on colonic bacterial community of mice was more extensively than MCC (IDF). Co-supplementation of the two fibers may help to increase the bacterial diversity and reduce the conditional pathogens in the colon of mice. |
| وصف الملف: | application/octet-stream; application/pdf |
| اللغة: | English |
| تدمد: | 1664-302X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::982bba18700260f52bce2cad8b1a4b93Test https://doi.org/10.3389/fmicb.2017.00966Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....982bba18700260f52bce2cad8b1a4b93 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1664302X |
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