Epithelial Sodium Channel-Mediated Sodium Transport Is Not Dependent on the Membrane-Bound Serine Protease CAP2/Tmprss4
| العنوان: | Epithelial Sodium Channel-Mediated Sodium Transport Is Not Dependent on the Membrane-Bound Serine Protease CAP2/Tmprss4 |
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| المؤلفون: | Julie Bapst, Camille Ansermet, Anna Keppner, Antoine Nobile, Ditte Andreasen, Anne-Marie Mérillat, Sumedha Malsure, Edith Hummler, Marc Maillard, Qing Wang |
| المصدر: | PLoS ONE, Vol 10, Iss 8, p e0135224 (2015) PLoS One PLoS ONE Plos One, vol. 10, no. 8, pp. e0135224 |
| بيانات النشر: | Public Library of Science (PLoS), 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Epithelial sodium channel, Proteases, medicine.medical_specialty, Sodium, Science, Absorption, Physicochemical, Animals, Biological Transport, Cell Membrane/metabolism, Epithelial Sodium Channels/metabolism, Gene Knockout Techniques, Homeostasis, Membrane Proteins/deficiency, Membrane Proteins/genetics, Mice, Serine Endopeptidases/deficiency, Serine Endopeptidases/genetics, Sodium/metabolism, chemistry.chemical_element, Biology, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, In vivo, Internal medicine, medicine, Epithelial Sodium Channels, 030304 developmental biology, Serine protease, 0303 health sciences, Multidisciplinary, Aldosterone, urogenital system, Cell Membrane, Serine Endopeptidases, Membrane Proteins, respiratory system, Endocrinology, chemistry, 030220 oncology & carcinogenesis, Knockout mouse, biology.protein, Medicine, hormones, hormone substitutes, and hormone antagonists, Research Article |
| الوصف: | The membrane-bound serine protease CAP2/Tmprss4 has been previously identified in vitro as a positive regulator of the epithelial sodium channel (ENaC). To study its in vivo implication in ENaC-mediated sodium absorption, we generated a knockout mouse model for CAP2/Tmprss4. Mice deficient in CAP2/Tmprss4 were viable, fertile, and did not show any obvious histological abnormalities. Unexpectedly, when challenged with sodium-deficient diet, these mice did not develop any impairment in renal sodium handling as evidenced by normal plasma and urinary sodium and potassium electrolytes, as well as normal aldosterone levels. Despite minor alterations in ENaC mRNA expression, we found no evidence for altered proteolytic cleavage of ENaC subunits. In consequence, ENaC activity, as monitored by the amiloride-sensitive rectal potential difference (ΔPD), was not altered even under dietary sodium restriction. In summary, ENaC-mediated sodium balance is not affected by lack of CAP2/Tmprss4 expression and thus, does not seem to directly control ENaC expression and activity in vivo. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9631bd63ec79b62e0f63c8203a22a042Test https://doaj.org/article/150db2d379c04a63818ede209b929fa0Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9631bd63ec79b62e0f63c8203a22a042 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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