Urocortin 1: A putative excitatory neurotransmitter in the enteric nervous system
| العنوان: | Urocortin 1: A putative excitatory neurotransmitter in the enteric nervous system |
|---|---|
| المؤلفون: | Ayako Kurematsu, Hajime Ariga, Shigeru Yamato, Tetsuya Ando, Tomofumi Amano, Gen Komaki, Keiji Wada |
| المصدر: | Neurogastroenterology & Motility. 32 |
| بيانات النشر: | Wiley, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Agonist, medicine.medical_specialty, Contraction (grammar), Colon, Physiology, medicine.drug_class, Muscarinic Antagonists, Enteric Nervous System, Rats, Sprague-Dawley, 03 medical and health sciences, chemistry.chemical_compound, Organ Culture Techniques, 0302 clinical medicine, Internal medicine, medicine, Animals, Antalarmin, Neurotransmitter, Urocortins, Neurotransmitter Agents, Dose-Response Relationship, Drug, Endocrine and Autonomic Systems, Chemistry, Gastroenterology, Antagonist, Electric Stimulation, Rats, Atropine, 030104 developmental biology, Endocrinology, Tetrodotoxin, Enteric nervous system, 030217 neurology & neurosurgery, Muscle Contraction, medicine.drug |
| الوصف: | Background Urocortin 1 (Ucn1), a stress-related peptide, is a member of the corticotropin-releasing factor (CRF) family and acts as a CRF1 receptor agonist. Ucn1 and CRF1 receptor immunoreactivity are present in the enteric nervous system (ENS), and Ucn1 elicits contraction of colonic muscle strips. Considering these findings, we have hypothesized that Ucn1 acts as an excitatory neurotransmitter in the ENS. The present study was conducted to determine whether exogenously applied Ucn1 causes contractions, whether it participates in neurally mediated contraction, and whether it is released from the ENS of the rat colon. Methods Isometric tension of the rat colonic muscle strips (middle to distal colon) in a longitudinal direction was measured. The effects of Ucn1 on phasic contractions were examined in the absence and presence of antalarmin (CRF1 receptor antagonist), tetrodotoxin (TTX), and atropine. The effects of antalarmin on electrical field stimulation (EFS)-induced contractions were examined in the absence and presence of atropine. Ucn1 peptide in the bath solution was measured after EFS using an EIA kit. Key results Ucn1 caused a significant and dose-dependent increase in phasic contractions. These effects were completely inhibited by antalarmin, TTX, and atropine. EFS-induced contractions were inhibited by antalarmin. Atropine markedly reduced EFS-induced contractions, and antalarmin did not decrease these contractions further. EFS elicited a significant increase in the concentration of Ucn1 in the bath solution, and this increase was completely inhibited by TTX. Conclusions and inferences These results suggest that Ucn1 acts as an excitatory neurotransmitter in the ENS enhancing the cholinergic neurotransmission. |
| تدمد: | 1365-2982 1350-1925 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::95c3b18a2674e4de347c31e17fa95e15Test https://doi.org/10.1111/nmo.13842Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....95c3b18a2674e4de347c31e17fa95e15 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13652982 13501925 |
|---|