Coupling azo dye degradation and biodiesel production by manganese-dependent peroxidase producing oleaginous yeasts isolated from wood-feeding termite gut symbionts
| العنوان: | Coupling azo dye degradation and biodiesel production by manganese-dependent peroxidase producing oleaginous yeasts isolated from wood-feeding termite gut symbionts |
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| المؤلفون: | Tamer Elsamahy, Jianzhong Sun, Mohamed El-Shetehy, Eleni Koutra, Rania Al-Tohamy, Sameh S. Ali, Michael Kornaros, Maha A. Khalil |
| المساهمون: | University of Manitoba |
| المصدر: | Biotechnology for Biofuels Biotechnology for Biofuels, Vol 14, Iss 1, Pp 1-25 (2021) |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0106 biological sciences, Wood-feeding termites, lcsh:Biotechnology, Oleaginous yeasts, Management, Monitoring, Policy and Law, 01 natural sciences, Applied Microbiology and Biotechnology, Textile azo dye, lcsh:Fuel, 03 medical and health sciences, chemistry.chemical_compound, Bioremediation, lcsh:TP315-360, 010608 biotechnology, lcsh:TP248.13-248.65, Debaryomyces hansenii, Phenol, Food science, Ligninases, 030304 developmental biology, 0303 health sciences, Biodiesel, biology, Renewable Energy, Sustainability and the Environment, Chemistry, Research, biology.organism_classification, Environmentally friendly, Yeast, General Energy, Wastewater, Biodiesel production, Lignin degradation inhibitors, Biotechnology |
| الوصف: | Background Textile industry represents one prevalent activity worldwide, generating large amounts of highly contaminated and rich in azo dyes wastewater, with severe effects on natural ecosystems and public health. However, an effective and environmentally friendly treatment method has not yet been implemented, while concurrently, the increasing demand of modern societies for adequate and sustainable energy supply still remains a global challenge. Under this scope, the purpose of the present study was to isolate promising species of yeasts inhabiting wood-feeding termite guts, for combined azo dyes and textile wastewater bioremediation, along with biodiesel production. Results Thirty-eight yeast strains were isolated, molecularly identified and subsequently tested for desired enzymatic activity, lipid accumulation, and tolerance to lignin-derived metabolites. The most promising species were then used for construction of a novel yeast consortium, which was further evaluated for azo dyes degradation, under various culture conditions, dye levels, as well as upon the addition of heavy metals, different carbon and nitrogen sources, and lastly agro-waste as an inexpensive and environmentally friendly substrate alternative. The novel yeast consortium, NYC-1, which was constructed included the manganese-dependent peroxidase producing oleaginous strains Meyerozyma caribbica, Meyerozyma guilliermondii, Debaryomyces hansenii, and Vanrija humicola, and showed efficient azo dyes decolorization, which was further enhanced depending on the incubation conditions. Furthermore, enzymatic activity, fatty acid profile and biodiesel properties were thoroughly investigated. Lastly, a dye degradation pathway coupled to biodiesel production was proposed, including the formation of phenol-based products, instead of toxic aromatic amines. Conclusion In total, this study might be the first to explore the application of MnP and lipid-accumulating yeasts for coupling dye degradation and biodiesel production. |
| وصف الملف: | application/pdf |
| تدمد: | 1754-6834 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9428d39cc2a3d925e7ffe99519dea0a1Test https://pubmed.ncbi.nlm.nih.gov/33685508Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9428d39cc2a3d925e7ffe99519dea0a1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17546834 |
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