Chitin-induced T6SS in Vibrio cholerae is dependent on ChiS activation
| العنوان: | Chitin-induced T6SS in Vibrio cholerae is dependent on ChiS activation |
|---|---|
| المؤلفون: | Asish K. Mukhopadhyay, Keinosuke Okamoto, Suman Das, Nabendu Sekhar Chatterjee, Debarpan Dhar, Rhishita Chourashi |
| المصدر: | Microbiology. 164:751-763 |
| بيانات النشر: | Microbiology Society, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Transcriptional Activation, 0301 basic medicine, Gene Transfer, Horizontal, Histidine Kinase, 030106 microbiology, Chitin, medicine.disease_cause, Microbiology, 03 medical and health sciences, chemistry.chemical_compound, Bacterial Proteins, Downregulation and upregulation, medicine, Secretion, Colonization, Vibrio cholerae, Gene, Microbial Viability, Chitinases, Gene Expression Regulation, Bacterial, Type VI Secretion Systems, Coculture Techniques, Transformation (genetics), chemistry, Horizontal gene transfer, Gene Deletion |
| الوصف: | Vibrio cholerae regularly colonizes the chitinous exoskeleton of crustacean shells in the aquatic region. The type 6 secretion system (T6SS) in V. cholerae is an interbacterial killing device. This system is thought to provide a competitive advantage to V. cholerae in a polymicrobial community of the aquatic region under nutrient-poor conditions. V. cholerae chitin sensing is known to be initiated by the activation of a two-component sensor histidine kinase ChiS in the presence of GlcNAc2 (N,N'-diacetylchitobiose) residues generated by the action of chitinases on chitin. It is known that T6SS in V. cholerae is generally induced by chitin. However, the effect of ChiS activation on T6SS is unknown. Here, we found that ChiS inactivation resulted in impaired bacterial killing and reduced expression of T6SS genes. Active ChiS positively affected T6SS-mediated natural transformation in V. cholerae. ChiS depletion or inactivation also resulted in reduced colonization on insoluble chitin surfaces. Therefore, we have shown that V. cholerae colonization on chitinous surfaces activates ChiS, which promotes T6SS-dependent bacterial killing and horizontal gene transfer. We also highlight the importance of chitinases in T6SS upregulation. |
| تدمد: | 1465-2080 1350-0872 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::93e8c0c62a81fa429b6881e5deb63ca7Test https://doi.org/10.1099/mic.0.000656Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....93e8c0c62a81fa429b6881e5deb63ca7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14652080 13500872 |
|---|