A Long Noncoding RNA ZEB1-AS1 Promotes Tumorigenesis and Predicts Poor Prognosis in Glioma
| العنوان: | A Long Noncoding RNA ZEB1-AS1 Promotes Tumorigenesis and Predicts Poor Prognosis in Glioma |
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| المؤلفون: | Meng-Long Fu, Hong-Hao Zhou, Shu-Hui Chen, Qiao-Li Lv, Bao Sun, Chen-Jie He, Chong-Zhen Qin, Lei Hu, Qiang Qu, Gui-Hua Wang |
| المصدر: | International Journal of Molecular Sciences; Volume 17; Issue 9; Pages: 1431 International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 17, Iss 9, p 1431 (2016) |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Carcinogenesis, Apoptosis, MMP9, Bioinformatics, lcsh:Chemistry, 0302 clinical medicine, glioma, Cyclin D1, prognostic biomarker, lcsh:QH301-705.5, lncRNA ZEB1-AS1, Spectroscopy, Gene knockdown, long non-coding RNA, Brain Neoplasms, General Medicine, Middle Aged, Cell cycle, Cadherins, Long non-coding RNA, Computer Science Applications, Matrix Metalloproteinase 9, 030220 oncology & carcinogenesis, Matrix Metalloproteinase 2, Female, RNA, Long Noncoding, Adult, Epithelial-Mesenchymal Transition, Biology, Article, Catalysis, Inorganic Chemistry, 03 medical and health sciences, Cell Line, Tumor, Glioma, Biomarkers, Tumor, medicine, Humans, Gene silencing, Epithelial–mesenchymal transition, Physical and Theoretical Chemistry, Molecular Biology, Cell Proliferation, Cyclin-Dependent Kinase 2, Organic Chemistry, Zinc Finger E-box-Binding Homeobox 1, medicine.disease, G1 Phase Cell Cycle Checkpoints, 030104 developmental biology, lcsh:Biology (General), lcsh:QD1-999, epithelial-mesenchymal transition, Cancer research |
| الوصف: | Emerging studies show that long noncoding RNAs (lncRNAs) have important roles in carcinogenesis. lncRNA ZEB1 antisense 1 (ZEB1-AS1) is a novel lncRNA, whose clinical significance, biological function, and underlying mechanism remains unclear in glioma. Here, we found that ZEB1-AS1 was highly expressed in glioma tissues, being closely related to clinical stage of glioma. Moreover, patients with high ZEB1-AS1 levels had poor prognoses, with the evidence provided by multivariate Cox regression analysis indicating that ZEB1-AS1 expression could serve as an independent prognostic factor in glioma patients. Functionally, silencing of ZEB1-AS1 could significantly inhibit cell proliferation, migration, and invasion, as well as promote apoptosis. Knockdown of ZEB1-AS1 significantly induced the G0/G1 phase arrest and correspondingly decreased the percentage of S phase cells. Further analysis indicated that ZEB1-AS1 could regulate the cell cycle by inhibiting the expression of G1/S transition key regulators, such as Cyclin D1 and CDK2. Furthermore, ZEB1-AS1 functioned as an important regulator of migration and invasion via activating epithelial to mesenchymal transition (EMT) through up-regulating the expression of ZEB1, MMP2, MMP9, N-cadherin, and Integrin-β1 as well as decreasing E-cadherin levels in the metastatic progression of glioma. Additionally, forced down-regulation of ZEB1-AS1 could dramatically promote apoptosis by increasing the expression level of Bax and reducing Bcl-2 expression in glioma. Taken together, our data suggest that ZEB1-AS1 may serve as a new prognostic biomarker and therapeutic target of glioma. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1422-0067 |
| DOI: | 10.3390/ijms17091431 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::935790aac1dcb6c1765a71544349eeebTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....935790aac1dcb6c1765a71544349eeeb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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| DOI: | 10.3390/ijms17091431 |