Differential expression of filamin B splice variants in giant cell tumor cells
| العنوان: | Differential expression of filamin B splice variants in giant cell tumor cells |
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| المؤلفون: | Kwok Chuen Wong, Joseph C.C. Tsui, Alex Chun Cheung, CP Lau, Stephen Kwok-Wing Tsui, Shekhar M. Kumta, Lin Huang |
| المصدر: | Oncology Reports |
| بيانات النشر: | Spandidos Publications, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Adult, Male, 0301 basic medicine, Cancer Research, Cell type, Adolescent, Filamins, Gene Expression, Bone Neoplasms, Core Binding Factor Alpha 1 Subunit, Filamin, Young Adult, 03 medical and health sciences, Biomarkers, Tumor, Tumor Cells, Cultured, medicine, Humans, Protein Isoforms, FLNA, FLNB, Giant Cell Tumor of Bone, biology, splicing variants, Articles, General Medicine, Middle Aged, Cell cycle, medicine.disease, Gene Expression Regulation, Neoplastic, 030104 developmental biology, Oncology, RANKL, Giant cell, Immunology, Cancer research, biology.protein, filamin B, Female, Giant-cell tumor of bone |
| الوصف: | Giant cell tumor of bone (GCT) is the most commonly reported non-malignant bone tumor in Hong Kong. This kind of tumor usually affects people aged 20-40 years. Also, it is well known for recurrence locally, especially when the tumor cannot be removed completely. Filamins are actin-binding proteins which contain three family members, filamin A, B and C. They are the products of three different genes, FLNA, FLNB and FLNC, which can generate various transcript variants in different cell types. In this study, we focused on the effects of FLNBv2 and FLNBv4 toward GCT cells. The only difference between FLNBv2 and FLNBv4 is that FLNBv4 does not contain hinge 1 region. We found that the relative abundance of FLNBv4 varies among different GCT cell lines while the expression level of FLNBv4 in normal osteoblasts was only marginally detectable. In the functional aspect, overexpression of FLNBv4 led to upregulation of RANKL, OCN, OPG and RUNX2, which are closely related to GCT cell survival and differentiation. Moreover, FLNBv4 can have a negative effect on cell viability of GCT cells when compare with FLNBv2. In conclusion, splicing variants of FLNB are differentially expressed in GCT cells and may play a role in the proliferation and differentiation of tumor cells. |
| تدمد: | 1791-2431 1021-335X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::92f19412eb2569653e4786f9fcb043edTest https://doi.org/10.3892/or.2016.5197Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....92f19412eb2569653e4786f9fcb043ed |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17912431 1021335X |
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