Serologic responses to the PfEMP1 DBL-CIDR head structure may be a better indicator of malaria exposure than those to the DBL-α tag
| العنوان: | Serologic responses to the PfEMP1 DBL-CIDR head structure may be a better indicator of malaria exposure than those to the DBL-α tag |
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| المؤلفون: | Philip L. Felgner, Rie Nakajima, Matthew B. Laurens, Amadou Niangaly, Christopher V. Plowe, Mahamadou A. Thera, Emily M Stucke, Jason A. Bailey, Albert E. Zhou, J. Alexandra Rowe, Abdoulaye K. Kone, Kirsten E. Lyke, Sonia Agrawal, Andrea A. Berry, Bourema Kouriba, Drissa Coulibaly, Amed Ouattara, Ogobara K. Doumbo, Shannon Takala-Harrison, Mark A. Travassos, Antoine Dara, Matthew Adams, Algis Jasinskas, Jozelyn Pablo, DeAnna J Friedman-Klabanoff |
| المصدر: | Malaria Journal, Vol 18, Iss 1, Pp 1-8 (2019) Stucke, E M, Niangaly, A, Berry, A A, Bailey, J A, Coulibaly, D, Ouattara, A, Lyke, K E, Laurens, M B, Dara, A, Adams, M, Pablo, J, Jasinskas, A, Nakajima, R, Zhou, A E, Agrawal, S, Friedman-Klabanoff, D J, Takala-Harrison, S, Kouriba, B, Kone, A K, Rowe, J A, Doumbo, O K, Felgner, P L, Thera, M A, Plowe, C V & Travassos, M A 2019, ' Serologic responses to the PfEMP1 DBL-CIDR head structure may be a better indicator of malaria exposure than those to the DBL-α tag ', Malaria Journal, vol. 18, no. 1, 273 . https://doi.org/10.1186/s12936-019-2905-9Test Malaria journal, vol 18, iss 1 Malaria Journal |
| بيانات النشر: | BMC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Protozoan Proteins, Microarray, Seroreactivity, Conserved sequence, 0302 clinical medicine, 030212 general & internal medicine, Malaria, Falciparum, Child, Conserved Sequence, Pediatric, Middle Aged, 3. Good health, seroreactivity, Infectious Diseases, Medical Microbiology, Child, Preschool, Protozoan, Public Health and Health Services, HIV/AIDS, Antibody, Infection, microarray, Falciparum, Adult, Protein Structure, lcsh:Arctic medicine. Tropical medicine, lcsh:RC955-962, 030231 tropical medicine, Plasmodium falciparum, Antigens, Protozoan, Biology, Microbiology, lcsh:Infectious and parasitic diseases, Young Adult, 03 medical and health sciences, var genes, Rare Diseases, Immune system, Antigen, Clinical Research, Tropical Medicine, parasitic diseases, medicine, Humans, lcsh:RC109-216, Antigens, Apical membrane antigen 1, Preschool, Research, Immunity, Infant, medicine.disease, biology.organism_classification, immunity, Virology, Protein Structure, Tertiary, Malaria, Vector-Borne Diseases, PfEMP1, Good Health and Well Being, Parasitology, biology.protein, Tertiary |
| الوصف: | Background Plasmodium falciparum erythrocyte membrane protein-1 (PfEMP1) antigens play a critical role in host immune evasion. Serologic responses to these antigens have been associated with protection from clinical malaria, suggesting that antibodies to PfEMP1 antigens may contribute to natural immunity. The first N-terminal constitutive domain in a PfEMP1 is the Duffy binding-like alpha (DBL-α) domain, which contains a 300 to 400 base pair region unique to each particular protein (the DBL-α “tag”). This DBL-α tag has been used as a marker of PfEMP1 diversity and serologic responses in malaria-exposed populations. In this study, using sera from a malaria-endemic region, responses to DBL-α tags were compared to responses to the corresponding entire DBL-α domain (or “parent” domain) coupled with the succeeding cysteine-rich interdomain region (CIDR). Methods A protein microarray populated with DBL-α tags, the parent DBL-CIDR head structures, and downstream PfEMP1 protein fragments was probed with sera from Malian children (aged 1 to 6 years) and adults from the control arms of apical membrane antigen 1 (AMA1) vaccine clinical trials before and during a malaria transmission season. Serological responses to the DBL-α tag and the DBL-CIDR head structure were measured and compared in children and adults, and throughout the season. Results Malian serologic responses to a PfEMP1’s DBL-α tag region did not correlate with seasonal malaria exposure, or with responses to the parent DBL-CIDR head structure in either children or adults. Parent DBL-CIDR head structures were better indicators of malaria exposure. Conclusions Larger PfEMP1 domains may be better indicators of malaria exposure than short, variable PfEMP1 fragments such as DBL-α tags. PfEMP1 head structures that include conserved sequences appear particularly well suited for study as serologic predictors of malaria exposure. Electronic supplementary material The online version of this article (10.1186/s12936-019-2905-9) contains supplementary material, which is available to authorized users. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1475-2875 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9284c1508af193912b0bea0838840f9fTest http://link.springer.com/article/10.1186/s12936-019-2905-9Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9284c1508af193912b0bea0838840f9f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14752875 |
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