Expression of CD304/neuropilin‐1 in adult b‐cell lymphoblastic leukemia/lymphoma and its utility for the measurable residual disease assessment
| العنوان: | Expression of CD304/neuropilin‐1 in adult b‐cell lymphoblastic leukemia/lymphoma and its utility for the measurable residual disease assessment |
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| المؤلفون: | Sumeet Gujral, Hasmukh Jain, Dhanlaxmi Shetty, Sachin Punatar, Manju Senger, Nilesh Deshpande, Nikhil Patkar, Anumeha Chaturvedi, Sitaram Ghogale, Papagudi Ganesan Subramanian, Karishma Girase, Anant Gokarn, Bhausaheb Bagal, Avinash Bonda, Navin Khattry, Vishesh Dudakia, Gaurav Chatterjee, Prashant Tembhare |
| المصدر: | International Journal of Laboratory Hematology. 43:990-999 |
| بيانات النشر: | Wiley, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Adult, Male, medicine.medical_specialty, Neoplasm, Residual, Adolescent, Coefficient of variation, Clinical Biochemistry, B-Cell Lymphoblastic Leukemia, 030204 cardiovascular system & hematology, Immunofluorescence, Gastroenterology, Flow cytometry, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Bone Marrow, Precursor B-Cell Lymphoblastic Leukemia-Lymphoma, hemic and lymphatic diseases, Internal medicine, Neuropilin 1, Biomarkers, Tumor, medicine, Humans, Aged, medicine.diagnostic_test, business.industry, Biochemistry (medical), Hematology, General Medicine, Middle Aged, medicine.disease, Neuropilin-1, Lymphoma, body regions, medicine.anatomical_structure, Female, Disease assessment, Bone marrow, business, 030215 immunology |
| الوصف: | INTRODUCTION Many new markers are being evaluated to increase the sensitivity and applicability of multicolor flow cytometry (MFC)-based measurable residual disease (MRD) monitoring. However, most of the studies are limited to childhood B-cell lymphoblastic leukemia/lymphoma (B-ALL), and reports in adult B-ALL are extremely scarce and limited to small cohorts. We studied the expression of CD304/neuropilin-1 in a large cohort of adult B-ALL patients and evaluated its practical utility in MFC-based MRD analysis. METHODS CD304 was studied in blasts from adult B-ALL patients and normal precursor B cells (NPBC) from non-B-ALL bone marrow samples using MFC. CD304 expression intensity and pattern were studied with normalized-mean fluorescent intensity (nMFI) and coefficient of variation of immunofluorescence (CVIF), respectively. MFC-based MRD was performed at end of induction (EOI; day-35), end of consolidation (EOC; day 78-80), and subsequent follow-up (SFU) time points. RESULTS CD304 was positive in 120/214(56.07%) and was significantly associated with BCR-ABL1 fusion (P = .001). EOI-MRD and EOC-MRD were positive in 129/214(60.3%) and 50/81(61.72%), respectively. CD304 was positive in a significant percentage of EOI (48%, 62/129) and EOC (52%, 26/50) MRD-positive B-ALL samples. Its expression was retained, lost, and gained in 73.7%, 26.3%, and 11.3% of EOI-MRD and 85.7%, 14.3%, and none of EOC-MRD samples, respectively. Low-level MRD ( |
| تدمد: | 1751-553X 1751-5521 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::91d3e2d960c36ca4f02cfd80547890f6Test https://doi.org/10.1111/ijlh.13456Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....91d3e2d960c36ca4f02cfd80547890f6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1751553X 17515521 |
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