High diagnostic accuracy for idiopathic Addison's disease with a sensitive radiobinding assay for autoantibodies against recombinant human 21-hydroxylase
| العنوان: | High diagnostic accuracy for idiopathic Addison's disease with a sensitive radiobinding assay for autoantibodies against recombinant human 21-hydroxylase |
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| المؤلفون: | S Laureti, G Casucci, F Santeusanio, Holger Luthman, Alberto Falorni, Anna-Lena Hulting, Andrej Nikoshkov, E Grenbäck, P Brunetti, Åke Lernmark |
| المصدر: | The Journal of Clinical Endocrinology & Metabolism. 80:2752-2755 |
| بيانات النشر: | The Endocrine Society, 1995. |
| سنة النشر: | 1995 |
| مصطلحات موضوعية: | Adult, Male, medicine.medical_specialty, Endocrinology, Diabetes and Metabolism, Clinical Biochemistry, morbo di Addison, Sensitivity and Specificity, Biochemistry, law.invention, Radioligand Assay, Endocrinology, Addison Disease, Reference Values, law, Internal medicine, Adrenal insufficiency, Humans, Medicine, Aged, Autoantibodies, Immunoassay, radioimmunoassay, biology, medicine.diagnostic_test, business.industry, Biochemistry (medical), Autoantibody, Radioimmunoassay, Middle Aged, medicine.disease, Recombinant Proteins, sensibilità diagnostica, Addison's disease, Acute Disease, Chronic Disease, biology.protein, Recombinant DNA, Female, Steroid 21-Hydroxylase, Radiobinding assay, Antibody, adrenolecuodistrofia, business, Anti-21-idrossilasi |
| الوصف: | Autoantibodies against 21-hydroxylase (P450c21) are common in idiopathic autoimmune Addison's disease. In the present work, we have developed a sensitive radiobinding assay using in vitro translated recombinant human 35S-P450c21. Levels of P450c21 antibodies (P450c21-Ab) were expressed as a relative index (P450c21 index) using a P450c21-Ab positive Addisonian serum and two antibody-negative healthy sera as positive and negative standards in healthy individuals. The upper level of normal was the mean + 3 SD. Positivity for P450c21-Ab was confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of immunoprecipitated 35S-P450c21. In 38 Addisonian patients, P450c21-Ab were found in 24/28 (86%) idiopathic, 0/5 post-tuberculosis, 0/3 adrenoleukodystrophy, and 0/2 post-adrenalectomy sera. Among healthy individuals, 1/70 (1.4%) were positive. The P450c21 index, as an estimate of P450c21-Ab levels, correlated inversely with the duration of idiopathic Addison's disease (r = -0.527; P = 0.007): 16/16 (100%) positive in patients with less than 20 yr and 8/12 (67%) positive in patients with more than 20 yr disease duration. The availability of this simple and sensitive radiobinding assay to evaluate levels of P450c21-Ab will permit large clinical studies as well as screening subjects at risk. In addition, the general population can now be screened to evaluate the predictive value of P450c21-Ab for Addison's disease. |
| تدمد: | 1945-7197 0021-972X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::91aaa00d809d0a3b94ba79912beff595Test https://doi.org/10.1210/jcem.80.9.7673419Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....91aaa00d809d0a3b94ba79912beff595 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19457197 0021972X |
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