Prokaryotic and Eukaryotic Fecal Microbiota in Irritable Bowel Syndrome Patients and Healthy Individuals Colonized With Blastocystis
| العنوان: | Prokaryotic and Eukaryotic Fecal Microbiota in Irritable Bowel Syndrome Patients and Healthy Individuals Colonized With Blastocystis |
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| المؤلفون: | Frédéric Delbac, Julie Brunet, Philippe Poirier, Michel Dapoigny, Céline Nourrisson, Julien Scanzi |
| المساهمون: | CHU Gabriel Montpied [Clermont-Ferrand], CHU Clermont-Ferrand, Laboratoire Microorganismes : Génome et Environnement (LMGE), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA) |
| المصدر: | Frontiers in Microbiology Frontiers in Microbiology, 2021, 12, ⟨10.3389/fmicb.2021.713347⟩ Frontiers in Microbiology, Vol 12 (2021) Frontiers in Microbiology, Frontiers Media, 2021, 12, ⟨10.3389/fmicb.2021.713347⟩ |
| بيانات النشر: | HAL CCSD, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Microbiology (medical), Mycobiota, [SDV]Life Sciences [q-bio], Gut flora, Microbiology, 18S ribosomal RNA, 03 medical and health sciences, medicine, Feces, Irritable bowel syndrome, Original Research, 030304 developmental biology, irritable bowel syndrome, 0303 health sciences, Tenericutes, Blastocystis, biology, gut microbiota, 030306 microbiology, 16S/18S ribosomal RNA gene analysis, biology.organism_classification, medicine.disease, eukaryome, QR1-502, 3. Good health, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, IBS-C, Ruminococcaceae |
| الوصف: | Blastocystis is the most frequently isolated protozoan from human stool. Its role in human health is still debated, and a high prevalence was reported in irritable bowel syndrome (IBS) subjects, suggesting a potential link with microbiota. In the present study, we aimed to investigate prokaryotic and eukaryotic microbiota in both IBS-C (constipated) and healthy individuals. We recruited 35 IBS-C patients and 23 healthy subjects, from which 12 and 11 carried Blastocystis, respectively. We performed 16S and 18S rRNA high-throughput sequencing on feces. Whereas we did not observe differences between infected and non-infected controls, several phyla were significantly modified in IBS-C patients according to the presence of Blastocystis. Tenericutes phylum and Ruminococcaceae family were especially increased in Blastocystis carriers. Furthermore, colonization with Blastocystis was associated with discrete changes in the microbial eukaryome, particularly among the Fungi taxa. Depending on the group of patients considered, the mycobiota changes do not go in the same direction and seem more deleterious in the IBS-C group. These results encourage further in vivo and in vitro investigations concerning the role of Blastocystis in the gut environment. |
| اللغة: | English |
| تدمد: | 1664-302X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::911aaee4069469a0663b99177cf10700Test https://hal.science/hal-03349938Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....911aaee4069469a0663b99177cf10700 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1664302X |
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