ChondrolectinMediates Growth Cone Interactions of Motor Axons with an Intermediate Target
| العنوان: | ChondrolectinMediates Growth Cone Interactions of Motor Axons with an Intermediate Target |
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| المؤلفون: | Ingolf Bach, Zhen Zhong, Jochen Ohnmacht, Thomas Becker, Michell M. Reimer, Catherina G. Becker |
| المصدر: | Zhong, Z, Ohnmacht, J, Reimer, M M, Bach, I, Becker, T & Becker, C G 2012, ' Chondrolectin mediates growth cone interactions of motor axons with an intermediate target ', Journal of Neuroscience, vol. 32, no. 13, pp. 4426-39 . https://doi.org/10.1523/JNEUROSCI.5179-11.2012Test Journal of Neuroscience, 32(13), 4426-4439. Washington, DC: Society for Neuroscience (2012). |
| بيانات النشر: | Society for Neuroscience, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Male, Growth Cones, LIM-Homeodomain Proteins, Multidisciplinary, general & others [F99] [Life sciences], Biochemistry, biophysics & molecular biology [F05] [Life sciences], Animals, Genetically Modified, Multidisciplinaire, généralités & autres [F99] [Sciences du vivant], medicine, Animals, Lectins, C-Type, Axon, Biochimie, biophysique & biologie moléculaire [F05] [Sciences du vivant], Growth cone, Chondrolectin, Zebrafish, Motor Neurons, Gene knockdown, biology, Gene Expression Profiling, General Neuroscience, Gene Expression Regulation, Developmental, Articles, Spinal muscular atrophy, Zebrafish Proteins, Motor neuron, biology.organism_classification, medicine.disease, Axons, medicine.anatomical_structure, nervous system, Gene Knockdown Techniques, Homeobox, Female, Genetics & genetic processes [F10] [Life sciences], Génétique & processus génétiques [F10] [Sciences du vivant], Neuroscience, Signal Transduction, Transcription Factors |
| الوصف: | The C-type lectin chondrolectin (chodl) represents one of the major gene products dysregulated in spinal muscular atrophy models in mice. However, to date, no function has been determined for the gene. We have identifiedchodland other novel genes potentially involved in motor axon differentiation, by expression profiling of transgenically labeled motor neurons in embryonic zebrafish. To enrich the profile for genes involved in differentiation of peripheral motor axons, we inhibited the function of LIM-HDs (LIM homeodomain factors) by overexpression of a dominant-negative cofactor, thereby rendering labeled axons unable to grow out of the spinal cord. Importantly, labeled cells still exhibited axon growth and most cells retained markers of motor neuron identity. Functional tests ofchodl, by overexpression and knockdown, confirm crucial functions of this gene for motor axon growthin vivo. Indeed, knockdown ofchodlinduces arrest or stalling of motor axon growth at the horizontal myoseptum, an intermediate target and navigational choice point, and reduced muscle innervation at later developmental stages. This phenotype is rescued bychodloverexpression, suggesting that correct expression levels ofchodlare important for interactions of growth cones of motor axons with the horizontal myoseptum. Combined, these results identify upstream regulators and downstream functions ofchodlduring motor axon growth. |
| وصف الملف: | application/pdf |
| تدمد: | 1529-2401 0270-6474 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::904205f1fef716fcbbe76c84ea0ab085Test https://doi.org/10.1523/jneurosci.5179-11.2012Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....904205f1fef716fcbbe76c84ea0ab085 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15292401 02706474 |
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