Charge substitution for a deep-pore residue reveals structural dynamics during BK channel gating
| العنوان: | Charge substitution for a deep-pore residue reveals structural dynamics during BK channel gating |
|---|---|
| المؤلفون: | Xixi Chen, Richard W. Aldrich |
| المصدر: | The Journal of General Physiology |
| بيانات النشر: | The Rockefeller University Press, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Membrane potential, BK channel, biology, Voltage-gated ion channel, Physiology, Chemistry, Inorganic chemistry, KcsA potassium channel, Ionic bonding, Gating, Article, Residue (chemistry), biology.protein, Biophysics, Humans, Large-Conductance Calcium-Activated Potassium Channels, Ion Channel Gating, Ion channel |
| الوصف: | The pore-lining amino acids of ion channel proteins reside on the interface between a polar (the pore) and a nonpolar environment (the rest of the protein). The structural dynamics of this region, which physically controls ionic flow, are essential components of channel gating. Using large-conductance, Ca2+-dependent K+ (BK) channels, we devised a systematic charge–substitution method to probe conformational changes in the pore region during channel gating. We identified a deep-pore residue (314 in hSlo1) as a marker of structural dynamics. We manipulated the charge states of this residue by substituting amino acids with different valence and pKa, and by adjusting intracellular pH. We found that the charged states of the 314 residues stabilized an open state of the BK channel. With models based on known structures of related channels, we postulate a dynamic rearrangement of the deep-pore region during BK channel opening/closing, which involves a change of the degree of pore exposure for 314. |
| اللغة: | English |
| تدمد: | 1540-7748 0022-1295 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8fba5a99021b02a063a8db43a893ba6eTest http://europepmc.org/articles/PMC3149437Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....8fba5a99021b02a063a8db43a893ba6e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15407748 00221295 |
|---|