Supramolecular Chitosan Micro-Platelets Synergistically Enhance Anti-Candida albicans Activity of Amphotericin B Using an Immunocompetent Murine Model
| العنوان: | Supramolecular Chitosan Micro-Platelets Synergistically Enhance Anti-Candida albicans Activity of Amphotericin B Using an Immunocompetent Murine Model |
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| المؤلفون: | Kawthar Bouchemal, Jean-Maurice Mallet, Christian Bories, Gilles Ponchel, Valérie Rouffiac, Martina Bombardi, Philippe M. Loiseau, Tiphany Grisin, Audrey Solgadi |
| المساهمون: | Stabilité génétique, Cellules Souches et Radiations (SCSR (U_967)), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Molécules bioactives, conception, isolement et synthèse (MBCIS), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Imagerie par Résonance Magnétique Médicale et Multi-Modalités (IR4M), Université Paris-Sud - Paris 11 (UP11)-Hôpital Bicêtre-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC), Laboratoire des biomolécules (LBM UMR 7203), Chimie Moléculaire de Paris Centre (FR 2769), École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Département de Chimie - ENS Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Physico-chimie, pharmacotechnie, biopharmacie (PCPB), Institut Galien Paris-Sud (IGPS), Université Paris-Sud - Paris 11 (UP11)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), Cellules Souches et Radiations (SCSR (U967 / UMR-E_008)), Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| المصدر: | Pharmaceutical Research Pharmaceutical Research, 2017, 34 (5), pp.1067-1082. ⟨10.1007/s11095-017-2117-3⟩ Pharmaceutical Research, American Association of Pharmaceutical Scientists, 2017, 34 (5), pp.1067-1082. ⟨10.1007/s11095-017-2117-3⟩ |
| بيانات النشر: | HAL CCSD, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Antifungal Agents, Swine, Chemistry, Pharmaceutical, [SDV]Life Sciences [q-bio], Pharmaceutical Science, 02 engineering and technology, Pharmacology, Chitosan, Mice, chemistry.chemical_compound, Amphotericin B, Amphotericin B deoxycholate, Candida albicans, Pharmacology (medical), ComputingMilieux_MISCELLANEOUS, Mice, Inbred BALB C, biology, Candidiasis, Hydrogels, 021001 nanoscience & nanotechnology, Corpus albicans, 3. Good health, Drug Combinations, Biochemistry, Self-healing hydrogels, Molecular Medicine, Female, 0210 nano-technology, Deoxycholic Acid, Biotechnology, medicine.drug, Blood Platelets, alpha-Cyclodextrins, 030106 microbiology, Poloxamer, macromolecular substances, 03 medical and health sciences, medicine, Animals, IC50, Mucous Membrane, Organic Chemistry, technology, industry, and agriculture, biology.organism_classification, In vitro, Disease Models, Animal, chemistry, Nanoparticles |
| الوصف: | The aim of this work is to design new chitosan conjugates able to self-organize in aqueous solution in the form of micrometer-size platelets. When mixed with amphotericin B deoxycholate (AmB-DOC), micro-platelets act as a drug booster allowing further improvement in AmB-DOC anti-Candida albicans activity. Micro-platelets were obtained by mixing oleoyl chitosan and α-cyclodextrin in water. The formulation is specifically-engineered for mucosal application by dispersing chitosan micro-platelets into thermosensitive pluronic® F127 20 wt% hydrogel. The formulation completely cured C. albicans vaginal infection in mice and had a superior activity in comparison with AmB-DOC without addition of chitosan micro-platelets. In vitro studies showed that the platelets significantly enhance AmB-DOC antifungal activity since the IC50 and the MIC90 decrease 4.5 and 4.8-times. Calculation of fractional inhibitory concentration index (FICI = 0.198) showed that chitosan micro-platelets act in a synergistic way with AmB-DOC against C. albicans. No synergy is found between spherical nanoparticles composed poly(isobutylcyanoacrylate)/chitosan and AmB-DOC. These results demonstrate for the first time the ability of flattened chitosan micro-platelets to have synergistic activity with AmB-DOC against C. albicans candidiasis and highlight the importance of rheological and mucoadhesive behaviors of hydrogels in the efficacy of the treatment. |
| اللغة: | English |
| تدمد: | 0724-8741 1573-904X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8f9c3c5ede888f314cd80dbfec8c5806Test https://hal.science/hal-02350871Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....8f9c3c5ede888f314cd80dbfec8c5806 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 07248741 1573904X |
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