Characterization and Functional Identification of a Gene Encoding Geranylgeranyl Diphosphate Synthase from Dunaliella bardawil
| العنوان: | Characterization and Functional Identification of a Gene Encoding Geranylgeranyl Diphosphate Synthase from Dunaliella bardawil |
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| المؤلفون: | Hong-Hao Jin, Jian-Guo Jiang, Ming-Hua Liang, Ying-Jie Liang |
| المصدر: | Journal of Agricultural and Food Chemistry. 63:7805-7812 |
| بيانات النشر: | American Chemical Society (ACS), 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Farnesyltranstransferase, Sequence Homology, Amino Acid, Amino Acid Motifs, Molecular Sequence Data, General Chemistry, Biology, Molecular biology, Protein Structure, Tertiary, Open Reading Frames, Open reading frame, Biochemistry, Rapid amplification of cDNA ends, Chlorophyta, Complementary DNA, Coding region, Amino Acid Sequence, Heterologous expression, Cloning, Molecular, General Agricultural and Biological Sciences, Peptide sequence, Gene, Plant Proteins |
| الوصف: | Geranylgeranyl diphosphate synthase (GGPS) catalyzes the biosynthesis of geranylgeranyl diphosphate, a key precursor for carotenoid biosynthesis. In this study, a full-length cDNA encoding GGPS from Dunaliella bardawil (DbGGPS) was isolated by rapid amplification of cDNA ends (RACE) for the first time. The full-length cDNA of DbGGPS was 1814 bp, containing a 1074 bp ORF encoding 357 amino acids with a calculated mass of 38.88 kDa. Analysis of DbGGPS genomic DNA revealed that it contained 10 exons and 9 introns. It was predicted that DbGGPS possessed a 48 amino acid transit peptide at its N terminus. Bioinformatic analysis revealed that DbGGPS was a member of a group of polyprenyltransferases with five conserved domains and two highly conserved aspartate-rich motifs. Using heterologous expression, carotenoid complementation assay, and gene deletion analysis, it was shown that the coding region of DbGGPS encodes a functional GGPS. This provides new gene sources for carotenoid genetic engineering. |
| تدمد: | 1520-5118 0021-8561 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8f29fe9aaf8019c25c9a9c079707177bTest https://doi.org/10.1021/acs.jafc.5b02732Test |
| رقم الانضمام: | edsair.doi.dedup.....8f29fe9aaf8019c25c9a9c079707177b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15205118 00218561 |
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