Anti-HPV16 E2 protein T-cell responses and viral control in women with usual vulvar intraepithelial neoplasia and their healthy partners
| العنوان: | Anti-HPV16 E2 protein T-cell responses and viral control in women with usual vulvar intraepithelial neoplasia and their healthy partners |
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| المؤلفون: | Micheline Moyal-Barracco, S. Berville, Monique Pelisse, Bénédicte Charmeteau, Pascale Villefroy, M.-F. Avril, Françoise Arnold, Françoise Plantier, S. Jacobelli, Antoine Touzé, Nicolas Dupin, Rémi Cheynier, M.O. North, T. Clerici, Suzanne Figueiredo, Jean-Gérard Guillet, Fedoua Sanaa, Isabelle Bourgault-Villada |
| المساهمون: | Institut Cochin (IC UM3 (UMR 8104 / U1016)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), AP-HP - Hôpital Cochin Broca Hôtel Dieu [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université Paris Descartes - Paris 5 (UPD5), Hôpital Ambroise Paré [AP-HP], Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), ProdInra, Migration, Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Recherche Agronomique (INRA)-Université de Tours |
| المصدر: | PLoS ONE PLoS ONE, Vol 7, Iss 5, p e36651 (2012) PLoS ONE, 2012, 7 (5), pp.e36651. ⟨10.1371/journal.pone.0036651⟩ PLoS ONE, Public Library of Science, 2012, 7 (5), pp.e36651. ⟨10.1371/journal.pone.0036651⟩ Plos One 5 (7), e36651. (2012) |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | CD4-Positive T-Lymphocytes, Male, Enzyme-Linked Immunospot Assay, Viral Diseases, Skin Neoplasms, medicine.medical_treatment, MALE SEXUAL PARTNERS, Gynecologic Infections, Virus Replication, 0302 clinical medicine, INFECTION, Interferon gamma, Longitudinal Studies, Prospective Studies, Immune Response, Skin Tumors, HUMAN-PAPILLOMAVIRUS TYPE-16, CERVICAL-CANCER, FUNCTIONAL SIGNATURES, CD4+T-CELL IMMUNITY, HPV16 E2, LESIONS, E7, E6, 0303 health sciences, Human papillomavirus 16, Immunity, Cellular, Multidisciplinary, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Vulvar Neoplasms, T Cells, ELISPOT, HPV infection, Middle Aged, 3. Good health, DNA-Binding Proteins, Cytokine, medicine.anatomical_structure, Infectious Diseases, Oncology, 030220 oncology & carcinogenesis, Medicine, Female, medicine.drug, Research Article, Interleukin 2, Adult, Skin Infections, Human Papillomavirus Infection, Clinical Research Design, T cell, Science, Immune Cells, Immunology, Sexually Transmitted Diseases, Médecine humaine et pathologie, Malignant Skin Neoplasms, Dermatology, Biology, 03 medical and health sciences, Interferon-gamma, medicine, Humans, 030304 developmental biology, Papillomavirus Infections, Cancers and Neoplasms, Oncogene Proteins, Viral, Vulvar intraepithelial neoplasia, medicine.disease, Virology, Viral replication, DNA, Viral, Interleukin-2, Human health and pathology, Clinical Immunology, Gynecological Tumors, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
| الوصف: | International audience; T-cell responses ( proliferation, intracellular cytokine synthesis and IFN gamma ELISPOT) against human papillomavirus 16 (HPV16) E2 peptides were tested during 18 months in a longitudinal study in eight women presenting with HPV16-related usual vulvar intraepithelial neoplasia (VIN) and their healthy male partners. In six women, anti-E2 proliferative responses and cytokine production ( single IFN gamma and/or dual IFN gamma/IL2 and/or single IL2) by CD4+ T lymphocytes became detectable after treating and healing of the usual VIN. In the women presenting with persistent lesions despite therapy, no proliferation was observed. Anti-E2 proliferative responses were also observed with dual IFN gamma/IL2 production by CD4+ T-cells in six male partners who did not exhibit any genital HPV-related diseases. Ex vivo IFN gamma ELISPOT showed numerous effector T-cells producing IFN gamma after stimulation by a dominant E2 peptide in all men and women. Since the E2 protein is absent from the viral particles but is required for viral DNA replication, these results suggest a recent infection with replicative HPV16 in male partners. The presence of polyfunctional anti-E2 T-cell responses in the blood of asymptomatic men unambiguously establishes HPV infection even without detectable lesions. These results, despite the small size of the studied group, provide an argument in favor of prophylactic HPV vaccination of young men in order to prevent HPV16 infection and viral transmission from men to women. |
| وصف الملف: | application/pdf |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8ea8196c4b2dad6856598dd1a42dae9eTest https://pubmed.ncbi.nlm.nih.gov/22590583Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....8ea8196c4b2dad6856598dd1a42dae9e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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