Copine 3 'CPNE3' is a novel regulator for insulin secretion and glucose uptake in pancreatic β-cells
| العنوان: | Copine 3 'CPNE3' is a novel regulator for insulin secretion and glucose uptake in pancreatic β-cells |
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| المؤلفون: | Shabana Anjum, Rania Saeed, Eman Abu-Gharbieh, Khuloud Bajbouj, Jalal Taneera, Abdul Khader Mohammed, Hema Unnikannan, Waseem El-Huneidi |
| المصدر: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-9 (2021) |
| بيانات النشر: | Nature Publishing Group UK, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Glucose uptake, medicine.medical_treatment, Science, Type 2 diabetes, Article, Insulin-Secreting Cells, Insulin Secretion, medicine, Gene silencing, Humans, Insulin, Cells, Cultured, Multidisciplinary, biology, Chemistry, Microarray analysis techniques, medicine.disease, Cell biology, Insulin receptor, Glucose, Diabetes Mellitus, Type 2, RNAi, NEUROD1, biology.protein, GLUT2, Medicine, Carrier Proteins |
| الوصف: | Copine 3 (CPNE3) is a calcium-dependent phospholipid-binding protein that has been found to play an essential role in cancer progression and stages. However, its role in pancreatic β-cell function has not been investigated. Therefore, we performed a serial of bioinformatics and functional experiments to explore the potential role of Cpne3 on insulin secretion and β-cell function in human islets and INS-1 (832/13) cells. RNA sequencing and microarray data revealed that CPNE3 is highly expressed in human islets compared to other CPNE genes. In addition, expression of CPNE3 was inversely correlated with HbA1c and reduced in human islets from hyperglycemic donors. Silencing of Cpne3 in INS-1 cells impaired glucose-stimulated insulin secretion (GSIS), insulin content and glucose uptake efficiency without affecting cell viability or inducing apoptosis. Moreover, mRNA and protein expression of the key regulators in glucose sensing and insulin secretion (Insulin, GLUT2, NeuroD1, and INSR) were downregulated in Cpne3-silenced cells. Taken together, data from the present study provides a new understanding of the role of CPNE3 in maintaining normal β-cell function, which might contribute to developing a novel target for future management of type 2 diabetes therapy. |
| اللغة: | English |
| تدمد: | 2045-2322 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8e4781629d2dc9ea1d1a412b4484ba2cTest http://europepmc.org/articles/PMC8526566Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....8e4781629d2dc9ea1d1a412b4484ba2c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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