Co-inhibition of Notch and NF-κB Signaling Pathway Decreases Proliferation through Downregulating IκB-α and Hes-1 Expression in Human Ovarian Cancer OVCAR-3 Cells
| العنوان: | Co-inhibition of Notch and NF-κB Signaling Pathway Decreases Proliferation through Downregulating IκB-α and Hes-1 Expression in Human Ovarian Cancer OVCAR-3 Cells |
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| المؤلفون: | Aynaz Mihanfar, Nosratollah Zarghami, Mohammad Rahmati-Yamchi, Bahman Yousefi, Effat Alizadeh, M Akbarzadeh, Maryam Majidinia |
| المصدر: | Drug Research. 67:13-19 |
| بيانات النشر: | Georg Thieme Verlag KG, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Small interfering RNA, Cell, Notch signaling pathway, Down-Regulation, Biology, 03 medical and health sciences, NF-KappaB Inhibitor alpha, Cell Line, Tumor, Nitriles, Drug Discovery, medicine, Humans, Sulfones, RNA, Small Interfering, Cell Proliferation, Receptors, Notch, Cell growth, NF-kappa B, Drug Synergism, Dipeptides, General Medicine, Transfection, I-kappa B Kinase, Cell biology, Reverse transcription polymerase chain reaction, 030104 developmental biology, medicine.anatomical_structure, Hes3 signaling axis, Cancer research, Transcription Factor HES-1, Signal transduction, Signal Transduction |
| الوصف: | Background: Ovarian cancer is one of the most lethal gynecological malignancies and numerous changes in signaling cascades are involved in the initiation and progression of ovarian cancerous cells. Here, we investigated the role of NF-κB and Notch pathways inhibition on human ovarian cancer OVCAR-3 cells proliferation and IκB-α and Hes-1 expression as 2 key genes in these pathways regulation. Methods: The effects of Bay 11-7085 and DAPT, NF-κB and Notch pathways specific inhibitors, on cell proliferation were evaluated using MTT assay. In addition, the cells were transfected by Notch and IKK-β siRNAs. mRNA and protein levels of target genes were measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and western blot after 48 h incubation with inhibitors and siRNAs. Results: Bay 11-7085 and DAPT significantly decreased the cell proliferation OVCAR-3. IκB-α and Hes-1 mRNA levels decreased to 5 or 3% and 6% or 2% after treatment with Bay 11-7085 or DAPT, respectively (p |
| تدمد: | 2194-9387 2194-9379 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8b5dd8ff4cdfdfedc46cc4dd4b0f9c65Test https://doi.org/10.1055/s-0042-115405Test |
| رقم الانضمام: | edsair.doi.dedup.....8b5dd8ff4cdfdfedc46cc4dd4b0f9c65 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 21949387 21949379 |
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