Roles of TGF-β/Smad signaling pathway in pathogenesis and development of gluteal muscle contracture
| العنوان: | Roles of TGF-β/Smad signaling pathway in pathogenesis and development of gluteal muscle contracture |
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| المؤلفون: | Yukun Ma, Honglei Zhang, Xiaopeng Tian, Tian You, Qi Zhu, Xintao Zhang, Wentao Zhang |
| المصدر: | Connective Tissue Research |
| بيانات النشر: | Informa UK Limited, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Adult, Male, medicine.medical_specialty, Contracture, Adolescent, PAI-1, Smad Proteins, SMAD, TGF-β/Smad pathway, Biology, Smad2/3, Biochemistry, Gluteal muscle contracture, Collagen Type I, Extracellular matrix, Young Adult, chemistry.chemical_compound, Rheumatology, Transforming Growth Factor beta, Internal medicine, Plasminogen Activator Inhibitor 1, medicine, Humans, Orthopedics and Sports Medicine, RNA, Messenger, Phosphorylation, Muscle, Skeletal, Gluteal muscles, Molecular Biology, Smad7, Cell Biology, Transforming growth factor beta, Immunohistochemistry, Up-Regulation, Collagen Type III, medicine.anatomical_structure, Endocrinology, chemistry, Plasminogen activator inhibitor-1, biology.protein, Buttocks, Female, medicine.symptom, Signal transduction, Research Article, Signal Transduction, Transforming growth factor |
| الوصف: | Purpose of the study Gluteal muscle contracture (GMC) is a chronic fibrotic disease of gluteal muscles which is characterized by excessive deposition of collagen in the extracellular matrix. Transforming growth factor (TGF)-βs have been shown to play an important role in the progression of GMC. However, the underlying mechanisms are not entirely clear. We sought to explore the expression of TGF-β/Smad pathway proteins and their downstream targets in gluteal muscle contracture disease. Materials and methods The expression levels of collagens type I/III, TGF-β1, Smad2/3/4/7 and PAI-1 (plasminogen activator inhibitor type 1) in gluteal muscle contraction (GMC) patients were measured using immunohistochemistry, reverse transcription and polymerase chain reaction (RT-PCR) and western blot assays. Results The expressions of collagens type I/III and TGF-β1 were significantly increased in the contraction band compared with unaffected muscle. In addition, R-Smad phosphorylation and Smad4 protein expression in the contraction band were also elevated, while the expression of Smad7 was significantly decreased in the fibrotic muscle of the GMC patients compared to the unaffected adjacent muscle. The protein and mRNA levels of PAI-1 were also remarkably increased in the contraction band compared with adjacent muscle. Immunohistochemical analysis also demonstrated that the expression levels of TGF-β1 and PAI-1 were higher in contraction band than those in the adjacent muscle. Conclusion Our data confirm the stimulating effects of the TGF-β/Smad pathway in gluteal muscle contracture disease and reveal the internal changes of TGF-β/Smad pathway proteins and their corresponding targets in gluteal muscle contracture patients. |
| تدمد: | 1607-8438 0300-8207 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8adcb8ba48c83338c0fcf04a2652fae1Test https://doi.org/10.3109/03008207.2014.964400Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....8adcb8ba48c83338c0fcf04a2652fae1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16078438 03008207 |
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