Human T-cell leukemia virus type I infects human lung epithelial cells and induces gene expression of cytokines, chemokines and cell adhesion molecules
| العنوان: | Human T-cell leukemia virus type I infects human lung epithelial cells and induces gene expression of cytokines, chemokines and cell adhesion molecules |
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| المؤلفون: | Akiko Miyazato, Jiro Fujita, Yuetsu Tanaka, Yoichiro Iwakura, Mariko Tomita, Satomi Yara, Maki Tamayose, Masachika Senba, Naoki Mori, Chie Ishikawa, Hiromitsu Teruya |
| المصدر: | Retrovirology, Vol 5, Iss 1, p 86 (2008) Retrovirology |
| بيانات النشر: | BMC, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | lcsh:Immunologic diseases. Allergy, Chemokine, Lymphocytosis, T-Lymphocytes, viruses, Adaptor Protein Complex 1, Enzyme-Linked Immunosorbent Assay, Mice, Transgenic, gag Gene Products, Human Immunodeficiency Virus, Cell Line, Proinflammatory cytokine, Mice, Proviruses, immune system diseases, hemic and lymphatic diseases, Virology, medicine, Animals, Humans, Lung, Human T-lymphotropic virus 1, biology, Reverse Transcriptase Polymerase Chain Reaction, Cell adhesion molecule, Gene Expression Profiling, Research, NF-kappa B, virus diseases, Epithelial Cells, Gene Products, tax, Flow Cytometry, medicine.disease, Coculture Techniques, Leukemia, Infectious Diseases, medicine.anatomical_structure, Cell culture, DNA, Viral, Immunology, biology.protein, Cytokines, medicine.symptom, Antibody, lcsh:RC581-607, Cell Adhesion Molecules |
| الوصف: | [Background] Human T-cell leukemia virus type I (HTLV-I) is associated with pulmonary diseases, characterized by bronchoalveolar lymphocytosis, which correlates with HTLV-I proviral DNA in carriers. HTLV-I Tax seems to be involved in the development of such pulmonary diseases through the local production of inflammatory cytokines and chemokines in T cells. However, little is known about induction of these genes by HTLV-I infection in lung epithelial cells. [Results] We tested infection of lung epithelial cells by HTLV-I by coculture studies in which A549 alveolar and NCI-H292 tracheal epithelial cell lines were cocultured with MT-2, an HTLV-I-infected T-cell line. Changes in the expression of several cellular genes were assessed by reverse transcription-polymerase chain reaction, enzyme-linked immunosorbent assay and flow cytometry. Coculture with MT-2 cells resulted in infection of lung epithelial cells as confirmed by detection of proviral DNA, HTLV-I Tax expression and HTLV-I p19 in the latter cells. Infection was associated with induction of mRNA expression of various cytokines, chemokines and cell adhesion molecule. NF-κB and AP-1 were also activated in HTLV-I-infected lung epithelial cells. In vivo studies showed Tax protein in lung epithelial cells of mice bearing Tax and patients with HTLV-I-related pulmonary diseases. [Conclusion] Our results suggest that HTLV-I infects lung epithelial cells, with subsequent production of cytokines, chemokines and cell adhesion molecules through induction of NF-κB and AP-1. These changes can contribute to the clinical features of HTLV-I-related pulmonary diseases. 論文 |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1742-4690 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8aaba95116ad30d72fdd8b54ea730bd5Test http://www.retrovirology.com/content/5/1/86Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....8aaba95116ad30d72fdd8b54ea730bd5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17424690 |
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