Involvement of endoplasmic reticulum stress and p53 in lncRNA MEG3-induced human hepatoma HepG2 cell apoptosis
| العنوان: | Involvement of endoplasmic reticulum stress and p53 in lncRNA MEG3-induced human hepatoma HepG2 cell apoptosis |
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| المؤلفون: | Meng‑Qi Xiang, Bin Liu, Ling‑Fei Wu, Li‑Xuan Liu, Jia‑Lin Feng, Zhen‑Lun Huang, Rui‑Pei Chen, Guo‑Ping Li |
| المصدر: | Oncology Reports. 36:1649-1657 |
| بيانات النشر: | Spandidos Publications, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Cancer Research, Carcinoma, Hepatocellular, Blotting, Western, Cell, Mice, Nude, Apoptosis, Biology, Mice, 03 medical and health sciences, 0302 clinical medicine, medicine, Animals, Humans, Endoplasmic Reticulum Chaperone BiP, Mice, Inbred BALB C, Reverse Transcriptase Polymerase Chain Reaction, ATF6, Cell growth, Endoplasmic reticulum, Liver Neoplasms, Hep G2 Cells, General Medicine, Cell cycle, Endoplasmic Reticulum Stress, Flow Cytometry, Immunohistochemistry, Molecular biology, 030104 developmental biology, medicine.anatomical_structure, Oncology, 030220 oncology & carcinogenesis, Unfolded protein response, Heterografts, RNA, Long Noncoding, Ectopic expression, Tumor Suppressor Protein p53 |
| الوصف: | Long non-coding RNAs (lncRNAs) play important roles in diverse biological processes. Although downregulation of lncRNA maternally expressed gene 3 (MEG3) has been identified in several types of cancers, little is known concerning its biological role and regulatory mechanism in hepatoma. Our previous studies demonstrated that MEG3 induces apoptosis in a p53-dependent manner. The aim of the present study was to determine whether endoplasmic reticulum (ER) stress is involved in MEG3‑induced apoptosis. Recombinant lentiviral vectors containing MEG3 (Lv‑MEG3) were constructed and transfected into HepG2 cells. A 3‑(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, RT‑PCR, flow cytometry, western blot analysis, immunofluorescence and immunohistochemistry were applied. Transfected HepG2 cells were also transplanted into nude mice, and the tumor growth curves were determined. The results showed that the recombinant lentivirus of MEG3 was transfected successfully into the HepG2 cells and the expression level of MEG3 was significantly increased. Ectopic expression of MEG3 inhibited HepG2 cell proliferation in vitro and in vivo, and also induced apoptosis. Ectopic expression of MEG3 increased ER stress‑related proteins 78‑kDa glucose‑regulated protein (GRP78), inositol‑requiring enzyme 1 (IRE1), RNA‑dependent protein kinase‑like ER kinase (PERK), activating transcription factor 6 (ATF6), C/EBP homologous protein (CHOP), caspase‑3, as well as p53 and NF‑κB expression accompanied by NF‑κB translocation from the cytoplasm to the nucleus. Furthermore, inhibition of NF‑κB with Bay11‑7082 decreased p53 expression in the MEG3‑transfected cells. These results indicate that MEG3 inhibits cell proliferation and induces apoptosis, partially via the activation of the ER stress and p53 pathway, in which NF‑κB signaling is required for p53 activation in ER stress. |
| تدمد: | 1791-2431 1021-335X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8a6654ba878db4798944bf1d5d784832Test https://doi.org/10.3892/or.2016.4919Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....8a6654ba878db4798944bf1d5d784832 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17912431 1021335X |
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