The TreaT-Assay: A Novel Urine-Derived Donor Kidney Cell-Based Assay for Prediction of Kidney Transplantation Outcome
| العنوان: | The TreaT-Assay: A Novel Urine-Derived Donor Kidney Cell-Based Assay for Prediction of Kidney Transplantation Outcome |
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| المؤلفون: | Thieme, Constantin J., Weist, Benjamin J. D., Mueskes, Annemarie, Roch, Toralf, Stervbo, Ulrik, Rosiewicz, Kamil, Wehler, Patrizia, Stein, Maik, Nickel, Peter, Kurtz, Andreas, Lachmann, Nils, Choi, Mira, Schmueck-Henneresse, Michael, Westhoff, Timm H., Reinke, Petra, Babel, Nina |
| المصدر: | Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019) Scientific Reports |
| بيانات النشر: | Freie Universität Berlin, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Adult, Graft Rejection, Male, T-Lymphocytes, lcsh:Medicine, kidney transplantation, Urine, Predictive markers, Kidney, Article, Prognostic markers, HLA Antigens, Humans, lcsh:Science, Cells, Cultured, donor, Aged, Kidney diseases, lcsh:R, Epithelial Cells, Translational research, Middle Aged, Tissue Donors, Kidney Tubules, Treatment Outcome, Biological Assay, Female, lcsh:Q, 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit, Spleen, Follow-Up Studies |
| الوصف: | Donor-reactive immunity plays a major role in rejection after kidney transplantation, but analysis of donor-reactive T-cells is not applied routinely. However, it has been shown that this could help to identify patients at risk of acute rejection. A major obstacle is the limited quantity or quality of the required allogenic stimulator cells, including a limited availability of donor-splenocytes or an insufficient HLA-matching with HLA-bank cells. To overcome these limitations, we developed a novel assay, termed the TreaT (Transplant reactive T-cells)-assay. We cultivated renal tubular epithelial cells from the urine of kidney transplant patients and used them as stimulators for donor-reactive T-cells, which we analyzed by flow cytometry. We could demonstrate that using the TreaT-assay the quantification and characterization of alloreactive T-cells is superior to other stimulators. In a pilot study, the number of pre-transplant alloreactive T-cells negatively correlated with the post-transplant eGFR. Frequencies of pre-transplant CD161+ alloreactive CD4+ T-cells and granzyme B producing alloreactive CD8+ T-cells were substantially higher in patients with early acute rejection compared to patients without complications. In conclusion, we established a novel assay for the assessment of donor-reactive memory T-cells based on kidney cells with the potential to predict early acute rejection and post-transplant eGFR. |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::89db1f01d22f9cd30ec0f26b57ab57e5Test https://doi.org/10.17169/refubium-26099Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....89db1f01d22f9cd30ec0f26b57ab57e5 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |