An evaluation of new gel matrices for the separation of PCR-amplified DNA fragments
| العنوان: | An evaluation of new gel matrices for the separation of PCR-amplified DNA fragments |
|---|---|
| المؤلفون: | Natalie Knight, C. S. Prakash, Ramanjini Gowda, Guohao He |
| المصدر: | Molecular Biotechnology. 5:63-65 |
| بيانات النشر: | Springer Science and Business Media LLC, 1996. |
| سنة النشر: | 1996 |
| مصطلحات موضوعية: | Electrophoresis, Gel matrix, Polyacrylamide, Bioengineering, Biology, Polymerase Chain Reaction, Applied Microbiology and Biotechnology, Biochemistry, law.invention, Matrix (mathematics), chemistry.chemical_compound, law, Molecular Biology, Polymerase chain reaction, Gel electrophoresis, Chromatography, DNA, Molecular biology, chemistry, DNA profiling, Evaluation Studies as Topic, Gels, Densitometry, Biotechnology |
| الوصف: | It has been shown that the novel gel matrix, PCR Purity Plus which consists of a vinyl-polymer of polyacrylamide, provides a superior and rapid means of separating DNA fragments. As this product has been discontinued, the two new commercial versions of this matrix (MDE® and GeneAmp®) with PCR Purity Plus were compared. Optimal conditions for resolving DNA fingerprint profiles for both matrices were defined. Both MDE and GeneAmp gels provided a clear separation of DNA fragments. However, the profiles obtained on GeneAmp gel were closest to that of PCR Purity Plus. These results should be useful to DNA fingerprinting studies where it is critical to obtain a clear resolution of complex DNA profiles. |
| تدمد: | 1559-0305 1073-6085 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::89cb99ece6226caac5c1607df33fd1b7Test https://doi.org/10.1007/bf02762413Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....89cb99ece6226caac5c1607df33fd1b7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15590305 10736085 |
|---|