Increased frequency of circulating CD19+CD24hiCD38hi B cells with regulatory capacity in patients with Ankylosing spondylitis (AS) naïve for biological agents
| العنوان: | Increased frequency of circulating CD19+CD24hiCD38hi B cells with regulatory capacity in patients with Ankylosing spondylitis (AS) naïve for biological agents |
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| المؤلفون: | Paloma Sánchez-Mateos, María-Eugenia Miranda-Carús, María-Belén Bautista-Caro, Chamaida Plasencia-Rodríguez, Diana Peiteado, Emilio Martín-Mola, Alejandro Villalba, Irene Monjo-Henry, Amaya Puig-Kröger, Eugenio de Miguel |
| المساهمون: | UAM. Departamento de Medicina, Instituto de Investigación Sanitaria Hospital Universitario de La Paz (IdiPAZ) |
| المصدر: | Repositorio Institucional de la Consejería de Sanidad de la Comunidad de Madrid Consejería de Sanidad de la Comunidad de Madrid Biblos-e Archivo. Repositorio Institucional de la UAM instname PLoS ONE PLoS ONE, Vol 12, Iss 7, p e0180726 (2017) |
| بيانات النشر: | Public Library of Science (PLoS), 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, B Cells, Physiology, Ankylosing Spondylitis, Frequency of circulating, lcsh:Medicine, CD38, White Blood Cells, Biological Factors, Spectrum Analysis Techniques, 0302 clinical medicine, Animal Cells, immune system diseases, Immune Physiology, hemic and lymphatic diseases, Medicine and Health Sciences, CD19+CD24hiCD38hi B cells, Disease activity, Enzyme-Linked Immunoassays, lcsh:Science, Innate Immune System, B-Lymphocytes, Multidisciplinary, biology, hemic and immune systems, Middle Aged, Flow Cytometry, Body Fluids, Blood, medicine.anatomical_structure, Spectrophotometry, Cytokines, Female, Tumor necrosis factor alpha, Cytophotometry, Cellular Types, Anatomy, Antibody, Research Article, Adult, Anti-TNFα drugs, Medicina, Immune Cells, Immunology, Antigens, CD19, Peripheral blood, Gastroenterology and Hepatology, Research and Analysis Methods, Peripheral blood mononuclear cell, CD19, Autoimmune Diseases, 03 medical and health sciences, Antigen, Breg cells, medicine, Humans, Spondylitis, Ankylosing, Antibody-Producing Cells, Immunoassays, Secretion, B cell, 030203 arthritis & rheumatology, Blood Cells, business.industry, lcsh:R, Inflammatory Bowel Disease, Biology and Life Sciences, CD24 Antigen, Cell Biology, Molecular Development, ADP-ribosyl Cyclase 1, 030104 developmental biology, Immune System, Immunologic Techniques, biology.protein, lcsh:Q, Clinical Immunology, Cytokine secretion, Clinical Medicine, Physiological Processes, business, Developmental Biology |
| الوصف: | Our objective was to study the frequency of circulating CD19+CD24hiCD38hi B cells (Breg) in AS patients. To this end, peripheral blood was drawn from AS patients naïve for TNF blockers (AS/nb) (n = 42) and healthy controls (HC) (n = 42). Six patients donated blood for a second time, 6 months after initiating treatment with anti-TNFα drugs. After isolation by Ficoll-Hypaque, PBMCs were stained with antibodies to CD3, CD4, CD19, CD24, and CD38, and examined by cytometry. For functional studies, total CD19+ B cells were isolated from PBMCs of 3 HC by magnetical sorting. Breg-depleted CD19+ B cells were obtained after CD19+CD24hiCD38hi B cells were removed from total CD19+ cells by cytometry. Total CD19+ B cells or Breg-depleted CD19+ B cells were established in culture and stimulated through their BCR. Secretion of IFNγ was determined by ELISA in culture supernatants. When compared with HC, AS/nb patients demonstrated a significantly increased frequency of Breg cells, which was independent of disease activity. Anti-TNFα drugs induced a significant reduction of circulating Breg numbers, which were no longer elevated after six months of treatment. Functional in vitro studies showed that the secretion of IFNγ was significantly higher in Breg-depleted as compared with total CD19+ B cells, indicating that Breg can downmodulate B cell pro-inflammatory cytokine secretion. In summary, an increased frequency of circulating CD19+CD24hiCD38hi B cells is observed in AS/nb patients, that is not related with disease activity; anti-TNFα drugs are able to downmodulate circulating Breg numbers in AS. This work was supported by Ministerio de Economía y Competitividad/Instituto de Salud Carlos III (MINECO/ISCIII) grant numbers FIS PI-16/01189, RD12/0009/0012 and RD16/0012/0012 (RIER, RETICS Program) (http://www.idi.mineco.gob.es/portal/site/MICINNTest, http://www.isciii.esTest), by Consejería de Educación de la Comunidad de Madrid/Fondo Europeo de Desarrollo Regional RAPHYME, grant number S2010/BMD-2350 (www.madrimasd.org) and by an unrestricted research grant from Roche Pharmaceuticals (www.roche.com) |
| وصف الملف: | application/pdf |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::89810234636b80aad3527851f6402f20Test https://doi.org/10.1371/journal.pone.0180726Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....89810234636b80aad3527851f6402f20 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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