Endothelin Receptor B2 (EDNRB2) Gene Is Associated with Spot Plumage Pattern in Domestic Ducks (Anas platyrhynchos)
| العنوان: | Endothelin Receptor B2 (EDNRB2) Gene Is Associated with Spot Plumage Pattern in Domestic Ducks (Anas platyrhynchos) |
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| المؤلفون: | Ling Li, Shijun Li, Xiuli Peng, Li Liu, Yanping Feng, Dan Li, Y. Gong |
| المصدر: | PLoS ONE PLoS ONE, Vol 10, Iss 5, p e0125883 (2015) |
| بيانات النشر: | Public Library of Science (PLoS), 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Candidate gene, Sequence analysis, Molecular Sequence Data, lcsh:Medicine, Biology, Polymorphism, Single Nucleotide, Chromosome Segregation, Complementary DNA, Genotype, Animals, Coding region, Amino Acid Sequence, lcsh:Science, Gene, Peptide sequence, Genetic Association Studies, Phylogeny, Skin, Genetics, Multidisciplinary, Base Sequence, Pigmentation, Receptors, Endothelin, Reproduction, lcsh:R, Feathers, Open reading frame, Ducks, Phenotype, Mutation, lcsh:Q, Research Article |
| الوصف: | Endothelin receptor B subtype 2 (EDNRB2) is a seven-transmembrane G-protein coupled receptor. In this study, we investigated EDNRB2 gene as a candidate gene for duck spot plumage pattern according to studies of chicken and Japanese quail. The entire coding region was cloned by the reverse transcription polymerase chain reaction (RT-PCR). Sequence analysis showed that duck EDNRB2 cDNA contained a 1311 bp open reading frame and encoded a putative protein of 436 amino acids residues. The transcript shared 89%-90% identity with the counterparts in other avian species. A phylogenetic tree based on amino acid sequences showed that duck EDNRB2 was evolutionary conserved in avian clade. The entire coding region of EDNRB2 were sequenced in 20 spot and 20 non-spot ducks, and 13 SNPs were identified. Two of them (c.940G>A and c.995G>A) were non-synonymous substitutions, and were genotyped in 647 ducks representing non-spot and spot phenotypes. The c.995G>A mutation, which results in the amino acid substitution of Arg332His, was completely associated with the spot phenotype: all 152 spot ducks were carriers of the AA genotype and the other 495 individuals with non-spot phenotype were carriers of GA or GG genotype, respectively. Segregation in 17 GA×GG and 22 GA×GA testing combinations confirmed this association since the segregation ratios and genotypes of the offspring were in agreement with the hypothesis. In order to investigate the underlying mechanism of the spot phenotype, MITF gene was used as cell type marker of melanocyte progenitor cells while TYR and TYRP1 gene were used as cell type markers of mature melanocytes. Transcripts of MITF, TYR and TYRP1 gene with expected size were identified in all pigmented skin tissues while PCR products were not obtained from non-pigmented skin tissues. It was inferred that melanocytes are absent in non-pigmented skin tissues of spot ducks. |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::88d4e4f033209934e3bbc27e2b6a7b80Test https://doi.org/10.1371/journal.pone.0125883Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....88d4e4f033209934e3bbc27e2b6a7b80 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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