Increased expression of blood muscarinic receptors in patients with reflex syncope
| العنوان: | Increased expression of blood muscarinic receptors in patients with reflex syncope |
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| المؤلفون: | Chris Adamopoulos, Alexandre Bolle, Charlie De Melo, Hugues Greney, Alban Simon, Catherine Schmidt-Mutter, Jacques Regnard, Erik Sauleau, Mickaël Schaeffer, Florian Zores, Magali Beaugey, Angelo Livolsi, Malika Bouhaddi, Maxime Beutelstetter, Gerald Roul, Nassim Dali-Youcef, Pascal Bousquet, Nathalie Niederhoffer, Pauline Helms |
| المساهمون: | CIC Strasbourg (Centre d’Investigation Clinique Plurithématique (CIC - P) ), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Nouvel Hôpital Civil de Strasbourg-Hôpital de Hautepierre [Strasbourg], Biopathologie de la Myéline, Neuroprotection et Stratégies Thérapeutiques (BMNST), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de cardiopédiatrie [Strasbourg], CHU Strasbourg, Laboratory of Neurobiology and Cardiovascular Pharmacology [Strasbourg], Fédération de Médecine Translationnelle de Strasbourg (FMTS), Université de Strasbourg (UNISTRA)-Université de Strasbourg (UNISTRA), Unit of Neonatal Intensive Care [Strasbourg], Service de cardiologie [Strasbourg], Groupe Médical Spécialisé Le Premium [Strasbourg], Laboratoire de Biochimie et de Biologie Moléculaire, CHU Strasbourg-Hôpital de Hautepierre [Strasbourg], Department of Functional Genomics and Cancer [Illkirch], Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Service de Physiologie et Exploration fonctionnelle [CHRU Besançon], Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Department of Public Health, methods in clinical research [Strasbourg], Université de Strasbourg (UNISTRA), This study was funded by grants from INSERM (https://www.inserm.frTest/) and FRM (https://www.frm.orgTest/) to PB, and grants from PHRC-I (http://www.girci-est.fr/animation/phrcinterregionalTest/) and Société Française de Cardiologie (https://www.sfcardio.frTest/) to AL. Additional funding was also received from Direction générale de l’offre de soins (DGOS), FFC and the University Hosptal of Strasbourg, France., Bodescot, Myriam, DALI-YOUCEF, Nassim, Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Strasbourg (UNISTRA)-Hôpital de Hautepierre [Strasbourg]-Nouvel Hôpital Civil de Strasbourg |
| المصدر: | PLoS ONE PLoS ONE, 2019, 14 (7), pp.e0219598. ⟨10.1371/journal.pone.0219598⟩ PLoS ONE, Public Library of Science, 2019, 14 (7), pp.e0219598. ⟨10.1371/journal.pone.0219598⟩ PLoS ONE, Vol 14, Iss 7, p e0219598 (2019) |
| بيانات النشر: | HAL CCSD, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Male, Physiology, [SDV]Life Sciences [q-bio], 030204 cardiovascular system & hematology, Pathology and Laboratory Medicine, Pediatrics, Biochemistry, chemistry.chemical_compound, 0302 clinical medicine, Reflexes, Muscarinic acetylcholine receptor, Syncope, Vasovagal, Medicine and Health Sciences, Child, Multidisciplinary, biology, Syncope (genus), Muscarinic acetylcholine receptor M2, Proteases, Receptors, Muscarinic, Acetylcholinesterase, Pathophysiology, Enzymes, Body Fluids, [SDV.MHEP.CSC] Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, [SDV] Life Sciences [q-bio], Blood, Physical Sciences, Cardiology, Medicine, Hyperexpression Techniques, Female, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Anatomy, Research Article, Signal Transduction, Adult, medicine.medical_specialty, Transmembrane Receptors, Science, Research and Analysis Methods, Syncope, 03 medical and health sciences, Signs and Symptoms, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, Diagnostic Medicine, 030225 pediatrics, Internal medicine, Gene Expression and Vector Techniques, medicine, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Humans, In patient, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, RNA, Messenger, [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Molecular Biology Techniques, Molecular Biology, Molecular Biology Assays and Analysis Techniques, business.industry, Biology and Life Sciences, Proteins, Cell Biology, Probability Theory, Probability Distribution, biology.organism_classification, chemistry, Acetylcholine Receptors, Enzymology, Reflex, Muscarinic Acetylcholine Receptors, Serine Proteases, Differential diagnosis, business, Mathematics, Neuroscience |
| الوصف: | International audience; Aims: Pathophysiology of reflex syncope is not fully understood but a vagal overactivity might be involved in this syncope. Previously, overexpression of muscarinic M2 receptors and acetylcholinesterase was found in particular in the heart and in lymphocytes of rabbits with vagal overactivity as well as in hearts of Sudden Infant Death Syndromes. The aim of this present study was to look at M2 receptor expression in blood of patients with reflex syncope. The second objective was to measure acetylcholinesterase expression in these patients.Methods and results: 136 subjects were enrolled. This monocenter study pooled 45 adults exhibiting recurrent reflex syncope compared with 32 healthy adult volunteers (18-50 years) and 38 children exhibiting reflex syncope requiring hospitalization compared with 21 controls (1-17 years). One blood sample was taken from each subject and blood mRNA expression of M2 receptors was assessed by qRT-PCR. Taking into account the non-symmetric distributions of values in both groups, statistical interferences were assessed using bayesian techniques. A M2 receptor overexpression was observed in adult and pediatric patients compared to controls. The medians [q1;q3] were 0.9 [0.3;1.9] in patients versus 0.2 [0.1;1.0] in controls; the probability that M2 receptor expression was higher in patients than in controls (Pr[patients>controls]) was estimated at 0.99. Acetylcholinesterase expression was also increased 0.7 [0.4;1.6] in patients versus 0.4 [0.2;1.1] in controls; the probability that acetylcholinesterase expression was higher in patients than in controls (Pr[patients>controls]) was estimated at 0.97. Both in adults and children, the expression ratio of M2 receptors over acetylcholinesterase was greater in the patient group compared with the control group.Conclusion: M2 receptor overexpression has been detected in the blood of both, adults and children, exhibiting reflex syncope. As in our experimental model, i.e. rabbits with vagal overactivity, acetylcholinesterase overexpression was associated with M2 receptor overexpression. For the first time, biological abnormalities are identified in vagal syncope in which only clinical signs are, so far, taken into account for differential diagnosis and therapeutic management. Further work will be needed to validate potential biomarkers of risk or severity associated with the cholinergic system. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::88c19f9fe145e618dd5303193c99b376Test https://www.hal.inserm.fr/inserm-02273017/file/journal.pone.0219598.pdfTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....88c19f9fe145e618dd5303193c99b376 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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