Isolation and characterization of a novel cDNA, UBAP1, derived from the tumor suppressor locus in human chromosome 9p21-22
| العنوان: | Isolation and characterization of a novel cDNA, UBAP1, derived from the tumor suppressor locus in human chromosome 9p21-22 |
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| المؤلفون: | Jieru Wang, Xiaorong Zhao, Ming Zhou, Jun Qian, Ke Tang, Guiyuan Li, Bicheng Zhang, Weifang Li, Zhaoyang Zeng, Xiaohui Zhang, Jianbo Yang, Shourong Shen |
| المصدر: | Journal of Cancer Research and Clinical Oncology. 127:613-618 |
| بيانات النشر: | Springer Science and Business Media LLC, 2001. |
| سنة النشر: | 2001 |
| مصطلحات موضوعية: | Cancer Research, DNA, Complementary, Molecular Sequence Data, Locus (genetics), Biology, Loss of heterozygosity, Mice, Complementary DNA, Animals, Humans, Coding region, Genes, Tumor Suppressor, Amino Acid Sequence, Northern blot, Gene, Expressed Sequence Tags, Genetics, Expressed sequence tag, Base Sequence, Reverse Transcriptase Polymerase Chain Reaction, Chromosome Mapping, Nasopharyngeal Neoplasms, General Medicine, Molecular biology, Gene nomenclature, Gene Expression Regulation, Oncology, Chromosomes, Human, Pair 9 |
| الوصف: | Purpose: To clone the putative tumor suppressor gene(s) in a refined region at 9p21–22 undergoing loss of heterozygosity in nasopharyngeal carcinoma (NPC). Methods: We systematically screened the expression patterns of 25 novel ESTs (expressed sequence tags) in a minimal common deleted region of 9p21–22 in NPC. One of these ESTs was found down-regulated in NPC. Subsequently, the corresponding gene sequence of this EST was established by cDNA cloning and RACE (rapid amplification of cDNA end) procedures. Furthermore, a mouse homologue of this gene was identified. The expression of this gene was examined using Northern blot or reverse transcription-polymerase chain reaction (RT-PCR) in various human and mouse tissues. A limited screen for mutation of coding sequence of this novel human gene was undertaken using RT-PCR and direct sequencing analysis. Results: A novel gene was cloned. This gene is a new member of the UBA domain family, so we named it UBAP1 for ubiquitin-associated protein 1 (HUGO Gene Nomenclature Committee-approved symbol). Northern blot and RT-PCR analysis demonstrate a ubiquitous pattern of gene expression in human and mouse tissues. The direct sequencing analysis of the coding region of hUBAP1 following RT-PCR failed to reveal any mutations in a preliminary screen of NPC cell line HNE1 and primary nasopharyngeal carcinoma samples. Conclusions: We cloned a novel gene UBAP1, which is highly conserved between human and mouse. Clearly, as a novel member of UBA domain protein family and taking its map location into account, a more extensive analysis is essential to establish whether subtle mutations are present in nasopharyngeal carcinomas. |
| تدمد: | 1432-1335 0171-5216 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8841492057e0162533f9eb69793250fdTest https://doi.org/10.1007/s004320100252Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....8841492057e0162533f9eb69793250fd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14321335 01715216 |
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