Qualitative and Quantitative Analysis of IDH1 Mutation in Progressive Gliomas by Allele-Specific qPCR and Western Blot Analysis
| العنوان: | Qualitative and Quantitative Analysis of IDH1 Mutation in Progressive Gliomas by Allele-Specific qPCR and Western Blot Analysis |
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| المؤلفون: | Gabriele Röhn, Roland Goldbrunner, Pantelis Stavrinou, Lena Dreher, Mohammad R. Toliat, Boris Krischek, Marco Timmer, Moritz Perrech |
| المصدر: | Technology in Cancer Research & Treatment |
| بيانات النشر: | SAGE Publications, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Adult, Male, Cancer Research, Blotting, Western, Biology, chemotherapy, Real-Time Polymerase Chain Reaction, DNA sequencing, law.invention, 03 medical and health sciences, Diffuse Glioma, 0302 clinical medicine, Western blot, isocitrate dehydrogenase 1, law, Glioma, medicine, Humans, Polymerase chain reaction, Alleles, 030304 developmental biology, 0303 health sciences, Messenger RNA, medicine.diagnostic_test, Brain Neoplasms, glioblastoma, sequencing, Middle Aged, medicine.disease, Molecular biology, Isocitrate Dehydrogenase, qPCR, Oncology, Mutation, Protein Expression Analysis, Immunohistochemistry, Original Article, Female, 030217 neurology & neurosurgery |
| الوصف: | To date, diagnosis of IDH1 mutation is based on DNA sequencing and immunohistochemistry, methods limited in terms of sensitivity and ease of use. Recently, the diagnosis of IDH1 mutation by real-time polymerase chain reaction was introduced as an alternative method. In this study, real-time polymerase chain reaction was validated as a tool for detection of IDH1 mutation, and expression levels were analyzed for correlation with course of the disease. A total of 113 tumor samples were obtained intraoperatively from 84 patients with glioma having a diagnosis of diffuse glioma (World Health Organization II), anaplastic glioma (World Health Organization III), secondary glioblastoma ± chemotherapy, primary glioblastoma ± chemotherapy (World Health Organization IV). Tumor samples were snap frozen and processed for sectioning and RNA and protein isolation. Presence of IDH1 mutation was determined by DNA sequencing. Hereafter, quantitative expression of IDH1 messenger RNA was assessed using real-time polymerase chain reaction with specific primers for IDH1 mutation and –wt; protein expression was verified by Western Blot analysis and immunohistochemistry. Additionally, 19 samples of low-grade glioma and their consecutive high-grade glioma were analyzed at different time points of the disease. IDH1 mutation was identified in 63% of samples by DNA sequencing. In correlation with the real-time polymerase chain reaction results, a cutoff value was determined. Above this threshold, sensitivity and specificity of real-time polymerase chain reaction in detecting IDH1 mutation were 98% and 94%, respectively. Quantitative analysis revealed that IDH1 mutation expression is upregulated in secondary glioblastoma (mean ± standard error of mean: 3.52 ± 0.55) compared to lower grade glioma (II = 1.54 ± 0.22; III = 1.67 ± 0.23). In contrast, IDH1 wt expression is upregulated in all glioma grades (concentration >0.1) compared to control brain tissue (0.007 ± 0.0016). Western Blot analysis showed a high concordance to both sequencing and real-time polymerase chain reaction results in qualitative analysis of IDH1 mutation status (specificity 100% and sensitivity 100%). Moreover, semiquantitative protein expression analysis also showed higher expression levels of mutated IDH1 in secondary glioblastoma. In our study, real-time polymerase chain reaction and Western Blot analysis were found to be highly efficient methods in detecting IDH1 mutation in glioma samples. As cost-effective and time-saving methods, real-time polymerase chain reaction and Western Blot analysis may therefore play an important role in IDH1 mutation analysis in the future. IDH1 mutation expression level was found to correlate with the course of disease to a certain extent. Yet, clinical factors as recurrent disease or prior radiochemotherapy did not alter IDH1 mutation expression level. |
| اللغة: | English |
| تدمد: | 1533-0338 1533-0346 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::87af7cd7bcc340f07d347e279c261932Test http://europepmc.org/articles/PMC6457076Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....87af7cd7bcc340f07d347e279c261932 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15330338 15330346 |
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