Establishment of the first World Health Organization International Genetic Reference Panel for quantitation of BCR-ABL mRNA
| العنوان: | Establishment of the first World Health Organization International Genetic Reference Panel for quantitation of BCR-ABL mRNA |
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| المؤلفون: | Dana Dvorakova, Martin C. Müller, Peter Rigsby, Nathalie Beaufils, Suzanne Kamel-Reid, Emmanuel Beillard, Timothy P. Hughes, Giuliana Romeo, Hakim El Housni, Dan Jones, Helen E. White, Andreas Hochhaus, Nicholas C.P. Cross, F. Lin, John M. Goldman, Jean Gabert, Lihui Wang, Y. Lynn Wang, Edmond S. K. Ma, Giuseppe Saglio, Katerina Zoi, Paul Matejtschuk, Stephen E. Langabeer, Hyun Gyung Goh, Richard D. Press, Dong-Wook Kim, Veli Kairisto, Susan Branford, Paul Metcalfe, Hans Ehrencrona, Dolors Colomer |
| المصدر: | Blood. 116:e111-e117 |
| بيانات النشر: | American Society of Hematology, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Standardization, Immunology, Fusion Proteins, bcr-abl, Computational biology, World Health Organization, Bioinformatics, Biochemistry, World health, Cell Line, 03 medical and health sciences, 0302 clinical medicine, Leukemia, Myelogenous, Chronic, BCR-ABL Positive, hemic and lymphatic diseases, medicine, Humans, RNA, Messenger, 030304 developmental biology, 0303 health sciences, Reverse Transcriptase Polymerase Chain Reaction, business.industry, Cell Biology, Hematology, Reference Standards, medicine.disease, 3. Good health, Leukemia, Real-time polymerase chain reaction, Mrna level, 030220 oncology & carcinogenesis, Health organization, business, Chronic myelogenous leukemia, K562 cells |
| الوصف: | Serial quantitation of BCR-ABL mRNA levels is an important indicator of therapeutic response for patients with chronic myelogenous leukemia and Philadelphia chromosome–positive acute lymphoblastic leukemia, but there is substantial variation in the real-time quantitative polymerase chain reaction methodologies used by different testing laboratories. To help improve the comparability of results between centers we sought to develop accredited reference reagents that are directly linked to the BCR-ABL international scale. After assessment of candidate cell lines, a reference material panel comprising 4 different dilution levels of freeze-dried preparations of K562 cells diluted in HL60 cells was prepared. After performance evaluation, the materials were assigned fixed percent BCR-ABL/control gene values according to the International Scale. A recommendation that the 4 materials be established as the first World Health Organization International Genetic Reference Panel for quantitation of BCR-ABL translocation by real-time quantitative polymerase chain reaction was approved by the Expert Committee on Biological Standardization of the World Health Organization in November 2009. We consider that the development of these reagents is a significant milestone in the standardization of this clinically important test, but because they are a limited resource we suggest that their availability is restricted to manufacturers of secondary reference materials. |
| تدمد: | 1528-0020 0006-4971 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::86cb6bde409f2f862c6e0119dd434a67Test https://doi.org/10.1182/blood-2010-06-291641Test |
| رقم الانضمام: | edsair.doi.dedup.....86cb6bde409f2f862c6e0119dd434a67 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15280020 00064971 |
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