Multiplexed analysis of glycan variation on native proteins captured by antibody microarrays
| العنوان: | Multiplexed analysis of glycan variation on native proteins captured by antibody microarrays |
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| المؤلفون: | Diane M. Simeone, Darren Hamelinck, Songming Chen, Tom LaRoche, Brian B. Haab, Randall E. Brand, Dean E. Brenner, Derek Bergsma |
| المصدر: | Nature Methods. 4:437-444 |
| بيانات النشر: | Springer Science and Business Media LLC, 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Glycan, Antibody microarray, Biochemistry, Antibodies, Antigen, Antigens, Neoplasm, Polysaccharides, Lectins, Humans, Molecular Biology, biology, Microarray analysis techniques, Glycobiology, Mucin-1, Mucin, Mucins, Transferrin, Cell Biology, Microarray Analysis, Carcinoembryonic Antigen, Pancreatic Neoplasms, carbohydrates (lipids), biology.protein, Antibody, DNA microarray, Protein Processing, Post-Translational, Biotechnology |
| الوصف: | Carbohydrate post-translational modifications on proteins are important determinants of protein function in both normal and disease biology. We have developed a method to allow the efficient, multiplexed study of glycans on individual proteins from complex mixtures, using antibody microarray capture of multiple proteins followed by detection with lectins or glycan-binding antibodies. Chemical derivatization of the glycans on the spotted antibodies prevented lectin binding to those glycans. Multiple lectins could be used as detection probes, each targeting different glycan groups, to build up lectin binding profiles of captured proteins. By profiling both protein and glycan variation in multiple samples using parallel sandwich and glycan-detection assays, we found cancer-associated glycan alteration on the proteins MUC1 and CEA in the serum of pancreatic cancer patients. Antibody arrays for glycan detection are highly effective for profiling variation in specific glycans on multiple proteins and should be useful in diverse areas of glycobiology research. |
| تدمد: | 1548-7105 1548-7091 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::867881934d612c946c17ee7d5e54c7cfTest https://doi.org/10.1038/nmeth1035Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....867881934d612c946c17ee7d5e54c7cf |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15487105 15487091 |
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