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Molecular cloning and characterization of the complementary DNA coding for the B-chain of murine Clq

التفاصيل البيبلوغرافية
العنوان: Molecular cloning and characterization of the complementary DNA coding for the B-chain of murine Clq
المؤلفون: Michael Loos, Franz Petry, Kenneth B.M. Reid
المصدر: FEBS Letters. (1):89-93
بيانات النشر: Published by Elsevier B.V.
مصطلحات موضوعية: mRNA, Molecular Sequence Data, Biophysics, Protein Sorting Signals, Molecular cloning, Biology, Biochemistry, Mice, Structural Biology, Sequence Homology, Nucleic Acid, Complementary DNA, Genetics, Animals, Humans, Coding region, Genomic library, RNA, Messenger, Northern blot, Cloning, Molecular, Promoter Regions, Genetic, Molecular Biology, Gene, Mice, Inbred BALB C, Messenger RNA, Complement C1q, Nucleic Acid Hybridization, RNA, DNA, RNA Probes, Cell Biology, Blotting, Northern, Molecular biology, Clq, Nucleotide sequence, Cloning
الوصف: cDNA clones coding for the B-chain of murine Clq were isolated from a mouse macrophage library. The characterized clones include the total coding region plus a leader sequence. High homology was found with human Clq B-chain in the coding region (81%). Northern blot analysis of total RNA from different tissues of Balb/c mice showed one band of approximately 1.2 kb. The highest signal was found in RNA preparations of thioglycolate-activated peritoneal macrophages. The probe also hybridized with mRNA from spleen, thymus and heart. Extremely weak signals were found in liver, kidney, lung and intestine tissues.
اللغة: English
تدمد: 0014-5793
DOI: 10.1016/0014-5793(89)81622-9
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::845fc212e15ecd623993914095100222Test
حقوق: OPEN
رقم الانضمام: edsair.doi.dedup.....845fc212e15ecd623993914095100222
قاعدة البيانات: OpenAIRE
الوصف
تدمد:00145793
DOI:10.1016/0014-5793(89)81622-9