miR‐148a inhibits early relapsed colorectal cancers and the secretion of VEGF by indirectly targeting HIF‐1α under non‐hypoxia/hypoxia conditions
| العنوان: | miR‐148a inhibits early relapsed colorectal cancers and the secretion of VEGF by indirectly targeting HIF‐1α under non‐hypoxia/hypoxia conditions |
|---|---|
| المؤلفون: | Jaw-Yuan Wang, I-Ping Yang, Hsiang-Lin Tsai, Yi-Ting Chen, Yung-Sung Yeh, Zhi-Feng Miao, Ching-Wen Huang |
| المصدر: | Journal of Cellular and Molecular Medicine |
| بيانات النشر: | John Wiley and Sons Inc., 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, Vascular Endothelial Growth Factor A, Colorectal cancer, Angiogenesis, chemistry.chemical_compound, 0302 clinical medicine, non‐hypoxia/hypoxia conditions, Medicine, Cells, Cultured, Tube formation, Neovascularization, Pathologic, HIF‐1α, Middle Aged, VEGF, Cell Hypoxia, Vascular endothelial growth factor, Blot, Gene Expression Regulation, Neoplastic, 030220 oncology & carcinogenesis, Molecular Medicine, Original Article, Female, medicine.symptom, colorectal cancers, Colorectal Neoplasms, HT29 Cells, Signal Transduction, 03 medical and health sciences, Cell Line, Tumor, Human Umbilical Vein Endothelial Cells, Humans, Secretion, Aged, business.industry, early relapse, Cell Biology, Original Articles, Hypoxia (medical), medicine.disease, HCT116 Cells, Hypoxia-Inducible Factor 1, alpha Subunit, digestive system diseases, MicroRNAs, 030104 developmental biology, chemistry, Cancer research, miR‐148a, Caco-2 Cells, Neoplasm Recurrence, Local, business |
| الوصف: | Vascular endothelial growth factor (VEGF) is correlated with angiogenesis and early relapse of colorectal cancer (CRC). This study investigated the role of miR‐148a in the regulation of VEGF/angiogenesis and early relapse of CRC. We established a stable clone with miR‐148a expression in HCT116 and HT29 cell lines and created a hypoxic condition by using CoCl2 to determine the underlying mechanism of miR‐148a. The effects of miR‐148a on the phosphoryl‐ERK (pERK)/hypoxia‐inducible factor‐1α (HIF‐1α)/VEGF pathway were evaluated through Western blotting and the inhibitory effect of miR‐148a on angiogenesis was demonstrated through a tube formation assay. Sixty‐three CRC tissues (28 early relapse and 35 non‐early relapse) were analysed to assess the relationship between miR‐148a and HIF‐1α/VEGF. The protein expression of pERK/HIF‐1α/VEGF in HCT116 and HT29 cells was significantly decreased by miR‐148a (all P |
| اللغة: | English |
| تدمد: | 1582-4934 1582-1838 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::82d6041d159e7142c59b13d90d33eb25Test http://europepmc.org/articles/PMC6484316Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....82d6041d159e7142c59b13d90d33eb25 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15824934 15821838 |
|---|