High-resolution Hi-C maps highlight multiscale 3D epigenome reprogramming during pancreatic cancer metastasis
| العنوان: | High-resolution Hi-C maps highlight multiscale 3D epigenome reprogramming during pancreatic cancer metastasis |
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| المؤلفون: | Yupei Zhao, Yuan Chen, Chengcheng Wang, Gang Yang, Ruiyuan Xu, Taiping Zhang, Huanyu Wang, Lei You, Xuning Fan, Bo Ren, Jinshou Yang |
| المصدر: | Journal of Hematology & Oncology, Vol 14, Iss 1, Pp 1-19 (2021) Journal of Hematology & Oncology |
| بيانات النشر: | BMC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Epigenomics, Cancer Research, Biology, Metastasis, Cell Line, Epigenesis, Genetic, 03 medical and health sciences, Epigenome, Mice, 0302 clinical medicine, Hi-C, Pancreatic cancer, Cell Line, Tumor, medicine, Tumor Cells, Cultured, Animals, Humans, Diseases of the blood and blood-forming organs, Epigenetics, Neoplasm Metastasis, Promoter Regions, Genetic, Molecular Biology, RC254-282, 030304 developmental biology, Regulation of gene expression, 0303 health sciences, Multi-omics, Research, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Hematology, medicine.disease, Chromatin, Gene Expression Regulation, Neoplastic, Pancreatic Neoplasms, Oncology, 030220 oncology & carcinogenesis, Cancer research, Female, RC633-647.5, Reprogramming |
| الوصف: | Background Pancreatic cancer’s poor prognosis is caused by distal metastasis, which is associated with epigenetic changes. However, the role of the 3D epigenome in pancreatic cancer biology, especially its metastasis, remains unclear. Methods Here, we developed high-resolution 3D epigenomic maps of cells derived from normal pancreatic epithelium, primary and metastatic pancreatic cancer by in situ Hi-C, ChIP-seq, ATAC-seq, and RNA-seq to identify key genes involved in pancreatic cancer metastasis Results We found that A/B compartments, contact domains, and chromatin loops changed significantly in metastatic pancreatic cancer cells, which are associated with epigenetic state alterations. Moreover, we found that upregulated genes, which were located in switched compartments, changed contact domains, and metastasis-specific enhancer-promoter loops, were related to cancer metastasis and poor prognosis of patients with pancreatic cancer. We also found that transcription factors in specific enhancer-promoter loop formation were also associated with metastasis. Finally we demonstrated that LIPC, looped to metastasis-specific enhancers, could promote pancreatic cancer metastasis. Conclusions These results highlight the multiscale 3D epigenome reprogramming during pancreatic cancer metastasis and expand our knowledge of mechanisms of gene regulation during pancreatic cancer metastasis. |
| اللغة: | English |
| تدمد: | 1756-8722 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::825775778a1e294db0f5837c6c647883Test https://doaj.org/article/956b886cd6c1454aa1dea1300677cdefTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....825775778a1e294db0f5837c6c647883 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17568722 |
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