Expression of a type B RIFIN in Plasmodium falciparum merozoites and gametes
| العنوان: | Expression of a type B RIFIN in Plasmodium falciparum merozoites and gametes |
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| المؤلفون: | David E. Arnot, Thomas Lavstsen, John Lusingu, Louise Turner, Thor G. Theander, Christian W. Wang, Bismarck Dinko, Dominique C Bengtsson, Colin J. Sutherland, Steven B. Mwakalinga |
| المصدر: | Malaria Journal, Vol 11, Iss 1, p 429 (2012) Mwakalinga, S B, Wang, C W, Bengtsson, D C, Turner, L, Dinko, B, Lusingu, J P, Arnot, D E, Sutherland, C J, Theander, T G & Lavstsen, T 2012, ' Expression of a type B RIFIN in Plasmodium falciparum merozoites and gametes ', Malaria Journal, vol. 11, no. 429, pp.-. https://doi.org/10.1186/1475-2875-11-429Test Malaria Journal |
| بيانات النشر: | BMC, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Adult, Male, lcsh:Arctic medicine. Tropical medicine, Protein family, Adolescent, Endemic Diseases, lcsh:RC955-962, Genes, Protozoan, Plasmodium falciparum, Protozoan Proteins, Antibodies, Protozoan, Asexual reproduction, Antigens, Protozoan, Host-Parasite Interactions, lcsh:Infectious and parasitic diseases, Young Adult, Antigenic variation, Parasite hosting, Animals, Humans, lcsh:RC109-216, Malaria, Falciparum, Child, Gene, Merozoite Surface Protein 1, biology, Base Sequence, Merozoites, Research, Gene Expression Regulation, Developmental, Infant, Membrane Proteins, DNA, Protozoan, Middle Aged, biology.organism_classification, Virology, Antigenic Variation, Infectious Diseases, Parasitology, Child, Preschool, Africa, biology.protein, Female, Antibody |
| الوصف: | Background The ability of Plasmodium falciparum to undergo antigenic variation, by switching expression among protein variants encoded by multigene families, such as var, rif and stevor, is key to the survival of this parasite in the human host. The RIFIN protein family can be divided into A and B types based on the presence or absence of a 25 amino acid motif in the semi-conserved domain. A particular type B RIFIN, PF13_0006, has previously been shown to be strongly transcribed in the asexual and sexual stages of P. falciparum in vitro. Methods Antibodies to recombinant PF13_0006 RIFIN were used in immunofluorescence and confocal imaging of 3D7 parasites throughout the asexual reproduction and sexual development to examine the expression of PF13_0006. Furthermore, reactivity to recombinant PF13_0006 was measured in plasma samples collected from individuals from both East and West African endemic areas. Results The PF13_0006 RIFIN variant appeared expressed by both released merozoites and gametes after emergence. 7.4% and 12.1% of individuals from East and West African endemic areas, respectively, carry plasma antibodies that recognize recombinant PF13_0006, where the antibody responses were more common among older children. Conclusions The stage specificity of PF13_0006 suggests that the diversity of RIFIN variants has evolved to provide multiple specialized functions in different stages of the parasite life cycle. These data also suggest that RIFIN variants antigenically similar to PF13_0006 occur in African parasite populations. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1475-2875 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::81d50b729b3e104dacb4ec9f70fd0c67Test http://www.malariajournal.com/content/11/1/429Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....81d50b729b3e104dacb4ec9f70fd0c67 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14752875 |
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