microRNA-181d associated with the methylation status of the MGMT gene in Glioblastoma multiforme cancer stem cells submitted to treatments with ionizing radiation and temozolomide
| العنوان: | microRNA-181d associated with the methylation status of the MGMT gene in Glioblastoma multiforme cancer stem cells submitted to treatments with ionizing radiation and temozolomide |
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| المؤلفون: | Gabriela Pereira-da-Silva, Caio M. Matias, Carlos Gilberto Carlotti, Fernanda Maris Peria, Daniela Pretti da Cunha Tirapelli, Felipe Amstalden Trevisan, Mucio Luiz de Assis Cirino, Andressa Romualdo Rodrigues, Fermino Sanches Lizarte Neto |
| المصدر: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, Methyltransferase, medicine.medical_treatment, Population, RADIAÇÃO IONIZANTE, 03 medical and health sciences, 0302 clinical medicine, Cancer stem cell, Radiation, Ionizing, microRNA, medicine, Biomarkers, Tumor, Temozolomide, Humans, education, Molecular Biology, DNA Modification Methylases, Aged, Aged, 80 and over, Chemotherapy, education.field_of_study, business.industry, Brain Neoplasms, General Neuroscience, Tumor Suppressor Proteins, DNA Methylation, Middle Aged, Prognosis, Radiation therapy, MicroRNAs, 030104 developmental biology, DNA Repair Enzymes, DNA methylation, Cancer research, Neoplastic Stem Cells, Female, Neurology (clinical), business, Glioblastoma, 030217 neurology & neurosurgery, Brazil, Developmental Biology, medicine.drug |
| الوصف: | Despite the increased understanding of the oncological mechanisms underlying Glioblastoma multiforme (GBM) pathophysiology, and recent advances in therapeutic strategies such as maximal surgical resection and post-operative radiotherapy with concomitant and adjuvant temozolomide chemotherapy, the prognosis for patients with brain tumors remains limited. Evidences indicate that the assessment of DNA methylation status in cancer stem cells would allow identifying molecules expressed in these cells, to lead to targeted elimination of this critical population from brain tumors, making the glioblastoma treatment more effective. This study aimed to analyze the role of microRNA-181d associated with the methylation status of the O6-methylguanine methyl transferase (MGMT) gene in Glioblastoma multiforme cancer stem cells subjected to treatment with temozolomide and ionizing radiation. Such responses were analyzed in terms of cell survival, evaluation of the MGMT gene methylation status by MS-HRM (Methylation-Sensitive High Resolution Melting), and analysis of miRNA-181d and MGMT gene expression by relative quantification of mRNA levels in cancer stem cells subjected to treatment with temozolomide and ionizing radiation, isolated or combined. We showed that ionizing radiation and temozolomide reduced the viability of cancer stem cells from GBM patients, as well as modified MGMT gene and miRNA-181d expression in cancer stem cells, suggesting that miRNA-181d interferes in the glioblastoma cancer stem cell response to treatment with temozolomide and ionizing radiation. |
| تدمد: | 1872-6240 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8115620fb4ee9b72eacf9d55def32ec2Test https://pubmed.ncbi.nlm.nih.gov/31226325Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....8115620fb4ee9b72eacf9d55def32ec2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18726240 |
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