Endo-pectinase Production by Bacillus pumilus NRRL B-212 and Optimization by RSM using Sugar Beet Pulp
| العنوان: | Endo-pectinase Production by Bacillus pumilus NRRL B-212 and Optimization by RSM using Sugar Beet Pulp |
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| المؤلفون: | Ozlem Tepe, Arzu Y. Dursun |
| المصدر: | Chemical and Biochemical Engineering Quarterly Volume 35 Issue 4 Chemical and Biochemical Engineering Quarterly, Vol 35, Iss 4, Pp 439-453 (2022) |
| بيانات النشر: | Croatian Society of Chemical Engineers/HDKI, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | response surface methodology, Chemical engineering, Process Chemistry and Technology, submerged fermentation, sugar beet pulp, food and beverages, enzyme production, TP155-156, General Chemistry, endo-pectinase, Bacillus pumilus NRRL B-212, bacillus pumilus nrrl b-212, Biochemistry |
| الوصف: | The present study aimed at optimization of culture conditions for endo-pectinase production by Bacillus pumilus NRRL B-212. In the first stage of the study, submerged fermentation experiments were performed to investigate effects of initial pH, carbon and nitrogen sources, salts and phosphate on endo-pectinase activity and maximum enzyme production was at pH: 8. The effect of initial pectin concentration on enzyme production was examined, and 1 % (w/v) pectin concentration was selected as the optimum pectin concentration. Yeast extract, (NH4)2SO4, and peptone were used as nitrogen sources, and the medium containing 0.05 % (w/v) ammonium sulphate was the medium where maximum activity was achieved. In the experiments investigating the effect of salts, the maximum activity value was determined in the medium containing 0.02 % NaCl. In addition, the effect of phosphate concentration on enzyme production was investigated, and the highest endo-pectinase activity was determined in medium containing 0.3 % K2HPO4 + 0.15 % KH2PO4. In the second stage of the study, solid-state fermentation studies were performed, and sugar beet pulp was used as agricultural waste. In order to obtain maximum endo-pectinase production and reveal the parameters influencing enzyme activity using sugar beet pulp, a Central Composite Design (CCD) was applied. The highest endo-pectinase activity was obtained as 147.75 U mL–1 in medium containing 6.78 % sugar beet pulp, 0.48 % (NH4)2SO4 and 0.12 % yeast extract. This work is licensed under a Creative Commons Attribution 4.0 International License. |
| وصف الملف: | application/pdf |
| تدمد: | 0352-9568 1846-5153 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::80e3df631cb096f373599e7cbfec3878Test https://doi.org/10.15255/cabeq.2021.1978Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....80e3df631cb096f373599e7cbfec3878 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 03529568 18465153 |
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