Supplementary Materials and Methods, Supplementary Figures 1 through 7, and Supplementary Tables 1 through 6 from HSulf-1 Modulates FGF2- and Hypoxia-Mediated Migration and Invasion of Breast Cancer Cells
التفاصيل البيبلوغرافية
العنوان:
Supplementary Materials and Methods, Supplementary Figures 1 through 7, and Supplementary Tables 1 through 6 from HSulf-1 Modulates FGF2- and Hypoxia-Mediated Migration and Invasion of Breast Cancer Cells
American Association for Cancer Research (AACR), 2023.
سنة النشر:
2023
الوصف:
Supplementary Materials and Methods. Figure S1: Quantitative Real-time PCR. Figure S2: MCF7 cells were transfected with HIF-1α siRNA oligos and control siRNA oligos before exposing them to hypoxia (3% oxygen) for indicated time intervals. Figure S3 : MTT assay was performed on MCF10DCIS batch clones after 24 hours of treatments as indicated. Figure S4: (A and B) pcDNA3.1 or pcDNA3.1 HSulf-1-myc/His plasmid transfected MCF10DCIS. Figure S5: K means cluster analysis of HSulf-1 expression with hypoxia signature genes as described in supplementary materials and methods. Figure S6: Kaplan-Meier survival analysis was performed to evaluate HSulf-1 mRNA expression levels with disease-free and overall patient survival. Figure S7: Proposed model. Table S1: Primers used for cloning, ChIP and siRNA oligos. Table S2: Patient Characteristics. Table S3: Chi-square statistical analysis of HSulf-1 and CAIX expression. Table S4: Prognostic factors in breast cancer cases compared with disease-free survival. Table S5: Prognostic factors in breast cancer cases compared with overall survival. Table S6: Characteristics of clinical parameters of breast cancer and HSulf-1 mRNA.
