Spermine-NBD as fluorescent probe for studies of the polyamine transport system in Leishmania donovani
| العنوان: | Spermine-NBD as fluorescent probe for studies of the polyamine transport system in Leishmania donovani |
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| المؤلفون: | Jean-Christophe Cintrat, Philippe M. Loiseau, Raphaël Labruère, Sébastien Pomel, Stéphanie Pethe, Elodie Jagu |
| المساهمون: | Biomolécules : Conception, Isolement, Synthèse (BioCIS), Institut de Chimie du CNRS (INC)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-CY Cergy Paris Université (CY) |
| المصدر: | Bioorganic & Medicinal Chemistry Letters Bioorganic & Medicinal Chemistry Letters, 2019, 29, pp.1710-1713. ⟨10.1016/j.bmcl.2019.05.030⟩ |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Clinical Biochemistry, Leishmania donovani, Pharmaceutical Science, Spermine, 01 natural sciences, Biochemistry, chemistry.chemical_compound, parasitic diseases, Drug Discovery, Polyamines, [CHIM]Chemical Sciences, Animals, Molecular Biology, IC50, Fluorescent Dyes, biology, Polyamine transport, 010405 organic chemistry, Organic Chemistry, Biological Transport, biology.organism_classification, Fluorescence, 3. Good health, 0104 chemical sciences, 010404 medicinal & biomolecular chemistry, chemistry, Molecular Medicine, Conjugate |
| الوصف: | This study describes the synthesis of fluorescent probes as potential substrates for the polyamine transport system (PTS) of Leishmania donovani. A competitive radioassay was used to determine the most efficient probe. We observed that the conjugate spermine-nitrobenzofurazan (Spm-NBD) was able to compete with [3H]-spermidine in L. donovani at a potent IC50 of 60 µM. |
| تدمد: | 1464-3405 0960-894X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7f2aaf090430132d327a65d2039cd073Test https://pubmed.ncbi.nlm.nih.gov/31122868Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7f2aaf090430132d327a65d2039cd073 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14643405 0960894X |
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