DNA methylation testing for endometrial cancer detection in urine, cervicovaginal self-samples and cervical scrapes
| العنوان: | DNA methylation testing for endometrial cancer detection in urine, cervicovaginal self-samples and cervical scrapes |
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| المؤلفون: | Birgit M. M. Wever, Rianne van den Helder, Annina P. van Splunter, Mignon D. J. M. van Gent, Jenneke C. Kasius, Johannes W. Trum, Harold R. Verhoeve, Wilhelmina M. van Baal, Alicia Hulbert, Lisanne Verhoef, Daniëlle A. M. Heideman, Birgit I. Lissenberg‐Witte, Nienke E. van Trommel, Renske D. M. Steenbergen, Maaike C. G. Bleeker |
| المساهمون: | Pathology, Obstetrics and gynaecology, Amsterdam Reproduction & Development (AR&D), CCA - Cancer biology and immunology, CCA - Imaging and biomarkers, Epidemiology and Data Science, APH - Methodology, AII - Cancer immunology, CCA - Cancer Treatment and quality of life, Obstetrics and Gynaecology, ARD - Amsterdam Reproduction and Development |
| المصدر: | International Journal of Cancer, 153(2), 341-351. Wiley-Liss Inc. International Journal of Cancer. Wiley-Liss Inc. Wever, B M M, van den Helder, R, van Splunter, A P, van Gent, M D J M, Kasius, J C, Trum, J W, Verhoeve, H R, van Baal, W M, Hulbert, A, Verhoef, L, Heideman, D A M, Lissenberg-Witte, B I, van Trommel, N E, Steenbergen, R D M & Bleeker, M C G 2023, ' DNA methylation testing for endometrial cancer detection in urine, cervicovaginal self-samples and cervical scrapes ', International Journal of Cancer, vol. 153, no. 2, pp. 341-351 . https://doi.org/10.1002/ijc.34504Test |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Cancer Research, DNA methylation, Oncology, cervical scrape, vaginal sample, endometrial cancer, urine |
| الوصف: | Endometrial cancer incidence is rising and current diagnostics often require invasive biopsy procedures. DNA methylation marker analysis of minimally- and non-invasive sample types could provide an easy-to-apply and patient-friendly alternative to determine cancer risk. Here, we compared the performance of DNA methylation markers to detect endometrial cancer in urine, cervicovaginal self-samples and clinician-taken cervical scrapes. Paired samples were collected from 103 patients diagnosed with stage I to IV endometrial cancer. Urine and self-samples were collected at home. All samples were tested for nine DNA methylation markers using quantitative methylation-specific PCR. Methylation levels measured in endometrial cancer patients were compared to unpaired samples of 317 healthy controls. Diagnostic performances were evaluated by univariable and multivariable logistic regression analysis, followed by leave-one-out cross-validation. Each methylation marker showed significantly higher methylation levels in all sample types of endometrial cancer patients compared to healthy controls (P |
| اللغة: | English |
| تدمد: | 0020-7136 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7e3d76b343730eed07e4060e72e29051Test https://research.vumc.nl/en/publications/304d8aee-ee00-490a-b163-021dc232ffe4Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7e3d76b343730eed07e4060e72e29051 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00207136 |
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