Early Diagnosis of Tuberculosis-Associated IgA Nephropathy with ESAT-6
| العنوان: | Early Diagnosis of Tuberculosis-Associated IgA Nephropathy with ESAT-6 |
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| المؤلفون: | Fu-Yun Sun, Hai-Ping Xu, Nan Zhao, Jing-Yi Sun |
| المصدر: | The Tohoku Journal of Experimental Medicine. 241:271-279 |
| بيانات النشر: | Tohoku University Medical Press, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Adult, Male, 0301 basic medicine, Tuberculosis, chemical and pharmacologic phenomena, Kidney, urologic and male genital diseases, General Biochemistry, Genetics and Molecular Biology, Nephropathy, Mycobacterium tuberculosis, 03 medical and health sciences, Bacterial Proteins, Antigen, medicine, Humans, Tuberculosis, Renal, Antigens, Bacterial, biology, business.industry, Glomerulonephritis, IGA, General Medicine, Middle Aged, respiratory system, bacterial infections and mycoses, medicine.disease, biology.organism_classification, Immunoglobulin A, Early Diagnosis, 030104 developmental biology, medicine.anatomical_structure, ESAT-6, Immunology, biology.protein, Immunohistochemistry, Female, Antibody, business, Acyltransferases |
| الوصف: | IgA nephropathy (IgAN) is the most common cause of primary renal diseases worldwide, and the early secreted antigenic target of 6 (ESAT-6) which was secreted by Mycobacterium tuberculosis (MTB) may be involved in the development and progression of IgAN. This study aimed to investigate the role of ESAT-6 for early diagnosis of IgAN caused by MTB infection. From 2011 to 2014, 21 patients with renal tuberculosis (RTB), 25 with IgAN, and 46 with IgAN infected with MTB (IgAN/MTB) were enrolled. Serum levels of antibodies against Mycobacterium tuberculosis antigen 85A (Ag85A) were measured by ELISA. Urine culture and phage amplified biologically assay were performed to detect MTB. HE staining was used to observe the morphological changes in kidney tissues. Immunohistochemistry was applied to detect the expression of ESAT-6. Immunofluorescence staining was conducted to detect IgA1. Positive rates of serum anti-Ag85A antibody and urine culture for MTB were higher in the RTB and IgAN/MTB groups than those in the IgAN group. The positive rates of plaques were also higher in RTB and IgAN/MTB groups than the positive rate in the IgAN group. By contrast, the positive rate of ESAT-6 was lower in the IgAN group than that in the RTB group or the IgAN/MTB group, whereas the expression levels of IgA1 were higher in the IgAN and IgAN/MTB groups, compared with the RTB group. Our findings suggest that ESAT-6 and IgA1 may be helpful for early diagnosis of IgAN caused by MTB infection. |
| تدمد: | 1349-3329 0040-8727 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7d295be79e0894675a625bf43969303fTest https://doi.org/10.1620/tjem.241.271Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7d295be79e0894675a625bf43969303f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13493329 00408727 |
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