Virulence Profiling and Molecular Typing of Shiga Toxin-Producing E. coli (STEC) from Human Sources in Brazil
| العنوان: | Virulence Profiling and Molecular Typing of Shiga Toxin-Producing E. coli (STEC) from Human Sources in Brazil |
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| المؤلفون: | Rodrigo T. Hernandes, Sandra R. S. Pinheiro, Samara Louzada Oliveira, Gabriela Rodrigues Francisco, Elizabeth Harummyy Takagi, Maria Cecilia Cergole-Novella, Érica de Lima Ori, Tânia Sueli de Andrade, Luis Fernando dos Santos, Adriene Maria Ferreira Cavalcanti, Beatriz E. C. Guth |
| المساهمون: | Adolfo Lutz Inst, Universidade Estadual Paulista (Unesp), Universidade Federal de São Paulo (UNIFESP) |
| المصدر: | Microorganisms, Vol 8, Iss 2, p 171 (2020) Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Microorganisms Volume 8 Issue 2 |
| بيانات النشر: | MDPI AG, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | stec, 0301 basic medicine, Microbiology (medical), Serotype, 030106 microbiology, Virulence, Biology, mlst, Microbiology, 03 medical and health sciences, fluids and secretions, Plasmid, Virology, Genotype, Pulsed-field gel electrophoresis, Typing, lcsh:QH301-705.5, Genetics, molecular typing, virulence markers, Pathogenicity island, STEC, serotypes, 030104 developmental biology, lcsh:Biology (General), bacteria, Multilocus sequence typing, MLST |
| الوصف: | Made available in DSpace on 2020-12-10T19:52:52Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-02-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Since no recent data characterizing Shiga toxin-producing E. coli (STEC) from human infections in Brazil are available, the present study aimed to investigate serotypes, stx genotypes, and accessory virulence genes, and also to perform pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) of 43 STEC strains recovered from 2007 to 2017. Twenty-one distinct serotypes were found, with serotype O111:H8 being the most common. However, serotypes less frequently reported in human diseases were also found and included a hybrid STEC/ETEC O100:H25 clone. The majority of the strains carried stxla as the sole stx genotype and were positive for the eae gene. Regarding the occurrence of 28 additional virulence genes associated with plasmids and pathogenicity islands, a diversity of profiles was found especially among the eae-harboring strains, which had combinations of markers composed of up to 12 distinct genes. Although PFGE analysis demonstrated genetic diversity between serotypes such as O157:H7, O111:H8, O26:H11, O118:H16, and O123:H2, high genetic relatedness was found for strains of serotypes O24:H4 and O145:H34. MLST allowed the identification of 17 distinct sequence types (STs) with ST 16 and 21 being the most common ones. Thirty-five percent of the strains studied were not typeable by the currently used MLST approach, suggesting new STs. Although STEC 0111:H8 remains the leading serotype in Brazil, a diversity of other serotypes, some carrying virulence genes and belonging to STs incriminated as causing severe disease, were found in this study. Further studies are needed to determine whether they have any epidemiological relevance. Adolfo Lutz Inst, Ctr Bacteriol, Natl Reference Lab STEC Infect & HUS, BR-01246000 Sao Paulo, SP, Brazil Univ Estadual Paulista, Dept Microbiol & Imunol, Inst Biociencias, BR-18618970 Botucatu, SP, Brazil Univ Fed Sao Paulo, Dept Microbiol, Imunol, Parasitol,Escola Paulista Med, BR-04023062 Sao Paulo, SP, Brazil Univ Estadual Paulista, Dept Microbiol & Imunol, Inst Biociencias, BR-18618970 Botucatu, SP, Brazil FAPESP: 2017/00411-1 FAPESP: 2014/20354-4 |
| وصف الملف: | application/pdf |
| تدمد: | 2076-2607 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7c6896618118e34929da555b9141a0ceTest https://doi.org/10.3390/microorganisms8020171Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7c6896618118e34929da555b9141a0ce |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20762607 |
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