High genetic diversity of Plasmodium falciparum in the low transmission setting of the Kingdom of Eswatini
| العنوان: | High genetic diversity of Plasmodium falciparum in the low transmission setting of the Kingdom of Eswatini |
|---|---|
| المؤلفون: | Maxwell Murphy, Nomcebo Mkhonta, Nomcebo Nhlabathi, Roly Gosling, Gugu Maphalala, Nyasatu Ntshalintshali, Simon Kunene, Bryan Greenhouse, Michelle S. Hsiang, Jordan Wilheim, Manik Saini, Sibonakaliso Vilakati, Lisa M. Prach, Michelle E. Roh, Sofonias K. Tessema, Anna Chen |
| المصدر: | The Journal of Infectious Diseases |
| بيانات النشر: | Cold Spring Harbor Laboratory, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Veterinary medicine, microsatellite genotyping, Multivariate analysis, 030231 tropical medicine, Plasmodium falciparum, Population, malaria, Population genetics, Biology, Logistic regression, Fixation index, law.invention, 03 medical and health sciences, Major Articles and Brief Reports, 0302 clinical medicine, Multiplicity of infection, Communicable Diseases, Imported, law, medicine, Immunology and Allergy, Humans, Parasites, Malaria, Falciparum, education, malaria elimination, education.field_of_study, Genetic diversity, Incidence, population genetics, Genetic Variation, DNA, Protozoan, medicine.disease, biology.organism_classification, 3. Good health, transmission intensity, 030104 developmental biology, Infectious Diseases, Transmission (mechanics), Epidemiological Monitoring, Microsatellite, Swaziland, parasite diversity, human activities, Eswatini, Genome, Protozoan, Malaria, Microsatellite Repeats |
| الوصف: | Background To better understand transmission dynamics, we characterized Plasmodium falciparum genetic diversity in Eswatini, where transmission is low and sustained by importation. Methods Twenty-six P. falciparum microsatellites were genotyped in 66% of confirmed cases (2014–2016; N = 582). Population and within-host diversity were used to characterize differences between imported and locally acquired infections. Logistic regression was used to assess the added value of diversity metrics to classify imported and local infections beyond epidemiology data alone. Results Parasite population in Eswatini was highly diverse (expected heterozygosity [HE] = 0.75) and complex: 67% polyclonal infections, mean multiplicity of infection (MOI) 2.2, and mean within-host infection fixation index (FWS) 0.84. Imported cases had comparable diversity to local cases but exhibited higher MOI (2.4 vs 2.0; P = .004) and lower mean FWS (0.82 vs 0.85; P = .03). Addition of MOI and FWS to multivariate analyses did not increase discrimination between imported and local infections. Conclusions In contrast to the common perception that P. falciparum diversity declines with decreasing transmission intensity, Eswatini isolates exhibited high parasite diversity consistent with high rates of malaria importation and limited local transmission. Estimates of malaria transmission intensity from genetic data need to consider the effect of importation, especially as countries near elimination. In contrast to the commonly held perception that P. falciparum diversity declines with decreasing transmission intensity, infections from Eswatini exhibited high parasite diversity consistent with high rates of malaria importation and limited local transmission. |
| وصف الملف: | application/pdf |
| تدمد: | 0022-1899 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b12e0d89963b6d2d6c8fd0180516269Test https://doi.org/10.1101/522896Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7b12e0d89963b6d2d6c8fd0180516269 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00221899 |
|---|