Thermodynamics, Kinetics and Mechanisms of Noncompetitive Allosteric Inhibition of Chymotrypsin by Dihydrolipoic Acid-Coated Gold Nanoclusters
| العنوان: | Thermodynamics, Kinetics and Mechanisms of Noncompetitive Allosteric Inhibition of Chymotrypsin by Dihydrolipoic Acid-Coated Gold Nanoclusters |
|---|---|
| المؤلفون: | Wen-Qi Chen, Miao-Miao Yin, Peng-Jun Song, Xiao-Hang He, Feng-Lei Jiang, Yi Liu |
| المصدر: | Langmuir. 36:6447-6457 |
| بيانات النشر: | American Chemical Society (ACS), 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Allosteric regulation, Kinetics, Metal Nanoparticles, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Nanomaterials, Nanoclusters, chemistry.chemical_compound, Dihydrolipoic acid, Electrochemistry, Chymotrypsin, General Materials Science, Spectroscopy, chemistry.chemical_classification, Thioctic Acid, biology, fungi, food and beverages, Surfaces and Interfaces, 021001 nanoscience & nanotechnology, Condensed Matter Physics, Combinatorial chemistry, 0104 chemical sciences, Enzyme, chemistry, biology.protein, Thermodynamics, Gold, 0210 nano-technology |
| الوصف: | Enzymes are an important class of biomacromolecules which catalyze many metabolic processes in living systems. Nanomaterials can be synthesized with tailored sizes as well as desired surface modifications, thus acting as promising enzyme regulators. Fluorescent gold nanoclusters (AuNCs) are a representative class of ultrasmall nanoparticles (USNPs) with sizes of ∼2 nm, smaller than most of proteins including enzymes. In this work, we chose α-chymotrypsin (ChT) and AuNCs as the model system. Activity assays and inhibition kinetics studies showed that dihydrolipoic acid (DHLA)-coated AuNCs (DHLA-AuNCs) had a high inhibitory potency ( |
| تدمد: | 1520-5827 0743-7463 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7a3406fa10b0df5a36632786d8087dceTest https://doi.org/10.1021/acs.langmuir.0c00699Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....7a3406fa10b0df5a36632786d8087dce |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15205827 07437463 |
|---|