Detrimental Role of CC Chemokine Receptor 4 in Murine Polymicrobial Sepsis
| العنوان: | Detrimental Role of CC Chemokine Receptor 4 in Murine Polymicrobial Sepsis |
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| المؤلفون: | Wolfram Kessler, Constanze Dassow, Marlene Mikulcak, Klaus Pfeffer, Pia Koerner, Ivo Steinmetz, Katrin Breitbach, K. Cziupka, Volker Assfalg, Stefan Maier, Tobias Traeger, Claus-Dieter Heidecke |
| المصدر: | Infection and Immunity. 76:5285-5293 |
| بيانات النشر: | American Society for Microbiology, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Chemokine, Receptors, CCR4, Immunology, CCR4, Peritonitis, Apoptosis, Biology, Microbiology, Proinflammatory cytokine, Sepsis, Mice, medicine, Animals, CCL17, Chemokine CCL22, Host Response and Inflammation, Reverse Transcriptase Polymerase Chain Reaction, Macrophages, medicine.disease, humanities, Mice, Inbred C57BL, Infectious Diseases, biology.protein, Female, Parasitology, Chemokine CCL17, Chemokines, CC chemokine receptors, CCL22 |
| الوصف: | CC chemokine receptor 4 (CCR4) and its two ligands, CCL17 and CCL22, are critically involved in different immune processes. In models of lipopolysaccharide-induced shock, CCR4-deficient (CCR4−/−) mice showed improved survival rates associated with attenuated proinflammatory cytokine release. Using CCR4−/−mice with a C57BL/6 background, this study describes for the first time the role of CCR4 in a murine model of polymicrobial abdominal sepsis, the colon ascendens stent peritonitis (CASP). CASP-induced sepsis led to a massive downregulation of CCR4 in lymphoid and nonlymphoid tissues, whereas the expression of CCL17 and CCL22 was independent of the presence of CCR4. After CASP, CCR4−/−animals showed a strongly enhanced bacterial clearance in several organs but not in the peritoneal lavage fluid and the blood. In addition, significantly reduced levels of proinflammatory cytokines/chemokines were measured in organ supernatants as well as in the sera of CCR4−/−mice. CCR4 deficiency consequently resulted in an attenuated severity of systemic sepsis and a strongly improved survival rate after CASP or CASP with intervention. Thus, our data provide clear evidence that CCR4 plays a strictly detrimental role in the course of polymicrobial sepsis. |
| تدمد: | 1098-5522 0019-9567 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::77dcaebb070a2dc6465a22436e30f420Test https://doi.org/10.1128/iai.00310-08Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....77dcaebb070a2dc6465a22436e30f420 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10985522 00199567 |
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