Type III Secretion System and Virulence Markers Highlight Similarities and Differences between Human- and Plant-Associated Pseudomonads Related to Pseudomonas fluorescens and P. putida
| العنوان: | Type III Secretion System and Virulence Markers Highlight Similarities and Differences between Human- and Plant-Associated Pseudomonads Related to Pseudomonas fluorescens and P. putida |
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| المؤلفون: | Victorien Decoin, Xavier Latour, Maïté Vicré-Gibouin, Corinne Barbey, Katy Jeannot, Alexandre Crépin, Dorian Bergeau, Annabelle Merieau, Sylvie Mazurier, Daniel Sperandio, Philippe Lemanceau, Patrick Plésiat |
| المساهمون: | Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC), Laboratoire de Microbiologie Signaux et Microenvironnement (LMSM), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Université de Franche-Comté (UFC), Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté ( UBFC ), Laboratoire de Microbiologie Signaux et Microenvironnement ( LMSM ), Université de Rouen Normandie ( UNIROUEN ), Normandie Université ( NU ) -Normandie Université ( NU ), Université de Franche-Comté ( UFC ), Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale ( Glyco-MEV ), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Université Bourgogne Franche-Comté [COMUE] (UBFC), Service de bactériologie [Besançon], Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Conseil Regional de Haute-Normandie & Ministere delegue a l'Enseignement Superieur et a la Recherche, Reseau VASI (GRR TERA), Groupement de Recherche CNRS Pseudomonas, IOBC-WPRS, FEDER |
| المصدر: | Applied and Environmental Microbiology Applied and Environmental Microbiology, American Society for Microbiology, 2015, 81 (7), pp.2579-2590. ⟨10.1128/AEM.04160-14⟩ Applied and Environmental Microbiology, American Society for Microbiology, 2015, 81 (7), pp.2579-2590. 〈10.1128/AEM.04160-14〉 |
| بيانات النشر: | American Society for Microbiology, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | POPULATION-DYNAMICS, Cystic Fibrosis, PATHOGENIC BACTERIA, Sequence Homology, Bacteremia, [ SDV.MP.BAC ] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, medicine.disease_cause, Polymerase Chain Reaction, Applied Microbiology and Biotechnology, CAROTOVORA SUBSP ATROSEPTICA, Type three secretion system, RNA, Ribosomal, 16S, Genotype, Cluster Analysis, Dictyostelium, Bacterial Secretion Systems, Respiratory Tract Infections, Phylogeny, Ecology, biology, Public and Environmental Health Microbiology, Temperature, GENOME SEQUENCE, Plants, GENOTYPIC DIVERSITY, 3. Good health, Pseudomonas aeruginosa, Biotechnology, DNA, Bacterial, Virulence Factors, MICROBIAL-CONTAMINATION, Molecular Sequence Data, Virulence, NUCLEOTIDE-SEQUENCES, Pseudomonas fluorescens, DNA, Ribosomal, Microbiology, medicine, Humans, Pseudomonas Infections, Plant Diseases, PV. TOMATO DC3000, DICTYOSTELIUM-DISCOIDEUM, Pathogenic bacteria, Sequence Analysis, DNA, biology.organism_classification, 16S ribosomal RNA, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, CLINICAL STRAIN, Bacteria, Food Science |
| الوصف: | Pseudomonas fluorescensis commonly considered a saprophytic rhizobacterium devoid of pathogenic potential. Nevertheless, the recurrent isolation of strains from clinical human cases could indicate the emergence of novel strains originating from the rhizosphere reservoir, which could be particularly resistant to the immune system and clinical treatment. The importance of type three secretion systems (T3SSs) in the relatedPseudomonas aeruginosanosocomial species and the occurrence of this secretion system in plant-associatedP. fluorescensraise the question of whether clinical isolates may also harbor T3SSs. In this study, isolates associated with clinical infections and identified in hospitals as belonging toP. fluorescenswere compared with fluorescent pseudomonads harboring T3SSs isolated from plants. Bacterial isolates were tested for (i) their genetic relationships based on their 16S rRNA phylogeny, (ii) the presence of T3SS genes by PCR, and (iii) their infectious potential on animals and plants under environmental or physiological temperature conditions. Two groups of bacteria were delineated among the clinical isolates. The first group encompassed thermotolerant (41°C) isolates from patients suffering from blood infections; these isolates were finally found to not belong toP. fluorescensbut were closely related and harbored highly conserved T3SS genes belonging to the Ysc-T3SS family, like the T3SSs fromP. aeruginosa. The second group encompassed isolates from patients suffering from cystic fibrosis; these isolates belonged toP. fluorescensand harbored T3SS genes belonging to the Hrp1-T3SS family found commonly in plant-associatedP. fluorescens. |
| تدمد: | 1098-5336 0099-2240 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::77cc2945d38018e5c18a39b3b251cf82Test https://doi.org/10.1128/aem.04160-14Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....77cc2945d38018e5c18a39b3b251cf82 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10985336 00992240 |
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