Human Wharton’s Jelly-Derived Mesenchymal Stromal Cells Primed by Tumor Necrosis Factor-α and Interferon-γ Modulate the Innate and Adaptive Immune Cells of Type 1 Diabetic Patients
| العنوان: | Human Wharton’s Jelly-Derived Mesenchymal Stromal Cells Primed by Tumor Necrosis Factor-α and Interferon-γ Modulate the Innate and Adaptive Immune Cells of Type 1 Diabetic Patients |
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| المؤلفون: | Mairvat Al- Mrahleh, Suzan Matar, Hanan Jafar, Suha Wehaibi, Nazneen Aslam, Abdalla Awidi |
| المصدر: | Frontiers in Immunology Frontiers in Immunology, Vol 12 (2021) |
| بيانات النشر: | Frontiers Media S.A., 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Adult, Male, Adolescent, Regulatory T cell, type 1 diabetes, T-Lymphocytes, Immunology, Cell Communication, Biology, Adaptive Immunity, Lymphocyte Activation, regulatory T cells, Wharton’s jelly-derived mesenchymal stromal cells, Immunomodulation, Interferon-gamma, Young Adult, Immune system, Downregulation and upregulation, Immunity, Pregnancy, Wharton's jelly, medicine, Immunology and Allergy, Humans, Wharton Jelly, priming, Cells, Cultured, Original Research, Cell Proliferation, Tumor Necrosis Factor-alpha, Mesenchymal stem cell, tolerogenic dendritic cells, FOXP3, Cell Differentiation, Mesenchymal Stem Cells, Dendritic Cells, RC581-607, Coculture Techniques, Immunity, Innate, Interleukin 10, medicine.anatomical_structure, Diabetes Mellitus, Type 1, Gene Expression Regulation, Cancer research, Female, Immunologic diseases. Allergy |
| الوصف: | The unique immunomodulation and immunosuppressive potential of Wharton’s jelly-derived mesenchymal stromal cells (WJ-MSCs) make them a promising therapeutic approach for autoimmune diseases including type 1 diabetes (T1D). The immunomodulatory effect of MSCs is exerted either by cell-cell contact or by secretome secretion. Cell-cell contact is a critical mechanism by which MSCs regulate immune-responses and generate immune regulatory cells such as tolerogenic dendritic cells (tolDCs) and regulatory T cell (Tregs). In this study, we primed WJ-MSCs with TNF-α and IFN-γ and investigated the immunomodulatory properties of primed WJ-MSCs on mature dendritic cells (mDCs) and activated T cells differentiated from mononuclear cells (MNCs) of T1D patient’s. Our findings revealed that primed WJ-MSCs impaired the antigen-mediated immunity, upregulated immune-tolerance genes and downregulated immune-response genes. We also found an increase in the production of anti-inflammatory cytokines and suppression of the production of pro-inflammatory cytokines. Significant upregulation of FOXP3, IL10 and TGFB1 augmented an immunosuppressive effect on adaptive T cell immunity which represented a strong evidence in support of the formation of Tregs. Furthermore, upregulation of many critical genes involved in the immune-tolerance mechanism (IDO1 and PTGES2/PTGS) was detected. Interestingly, upregulation of ENTPD1/NT5E genes express a strong evidence to switch immunostimulatory response toward immunoregulatory response. We conclude that WJ-MSCs primed by TNF-α and IFN-γ may represent a promising tool to treat the autoimmune disorders and can provide a new evidence to consider MSCs- based therapeutic approach for the treatment of TID. |
| اللغة: | English |
| تدمد: | 1664-3224 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::768c359149fa0ca19e8985b45f08795dTest http://europepmc.org/articles/PMC8506215Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....768c359149fa0ca19e8985b45f08795d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16643224 |
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